{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T01:09:15Z","timestamp":1773018555122,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T00:00:00Z","timestamp":1766880000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T00:00:00Z","timestamp":1766880000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100013501","name":"CNIB","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100013501","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004794","name":"Centre National de la Recherche Scientifique","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Cancer\u2010related sarcopenia (CRS) is a significant complication of head and neck carcinoma (HNC), characterised by muscle degeneration and poor clinical outcomes. Although various dietary and therapeutic interventions have been explored, most of them remain empirical, and the molecular mechanisms underlying CRS are not yet fully understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Transcription profiles of muscle fragments from 29 HNC patients and 8 control donors were analysed by bulk RNA sequencing (6\/29 and 3\/8) and\/or RT\u2010qPCR (29\/29 and 5\/8). In parallel, differentiating human myoblasts (AB1190) were subjected to indirect co\u2010culture with two types of effector cells: HNC cells (FaDu) or control epithelial cells (NHEK). The contactless effects of effector cells on target myoblasts were investigated using cell imaging to assess muscular differentiation, RT\u2010qPCR and Western blot to assess gene expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Bulk RNA sequencing identified 789 differentially expressed transcripts between HNC and control samples. Subsequent RT\u2010qPCR analysis focused on\n                      <jats:italic>IL32<\/jats:italic>\n                      and\n                      <jats:italic>BIRC3<\/jats:italic>\n                      mRNAs (up\u2010regulated in HNC samples) and\n                      <jats:italic>ACE1<\/jats:italic>\n                      mRNA (down\u2010regulated). Among male HNC patients, the\n                      <jats:italic>IL32\/ACE1<\/jats:italic>\n                      mRNA ratio was significantly elevated in CRS cases (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0001, effect size\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.57) and correlated with the severity of muscle atrophy (negative correlation with the Skeletal Muscle Index at a threshold of 10%:\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.093,\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u2009\u22120.41). In contrast, no such trend was observed for the\n                      <jats:italic>BIRC3\/ACE1<\/jats:italic>\n                      ratio. Exposure of human myoblasts to HNC cells induced inhibition of myogenesis and strong up\u2010regulation of IL32 mRNA and protein. In contrast, these effects were absent or much smaller under exposure to NHEK controls.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>IL32 is a potential biomarker for CRS in HNC patients. In addition, the HNC\u2013myoblast co\u2010cultivation model provides a promising in\u00a0vitro system to study CRS mechanisms, potentially reducing the reliance on animal models.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.70160","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T05:10:06Z","timestamp":1766985006000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enhanced Expression of\n                    <i>IL32<\/i>\n                    mRNA in Skeletal Muscles in the Context of Head and Neck Carcinomas"],"prefix":"10.1002","volume":"17","author":[{"given":"Imane","family":"Ba\u00efche","sequence":"first","affiliation":[{"name":"CNRS UMR 9018\u2010METSY Gustave Roussy and Universit\u00e9 Paris\u2010Saclay  Villejuif 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