{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T16:24:05Z","timestamp":1791822245449,"version":"4.3.4"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T00:00:00Z","timestamp":1769299200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T00:00:00Z","timestamp":1769299200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Skeletal muscle is essential for metabolic health and physical function. While resistance training promotes muscle hypertrophy, alternative therapeutic strategies are needed for individuals unable to engage in physical activity. Because beta\n                      <jats:sub>2<\/jats:sub>\n                      \u2010adrenergic stimulation induces muscle growth without mechanical load, we assessed muscle fibre type\u2013specific proteomic adaptations to prolonged beta\n                      <jats:sub>2<\/jats:sub>\n                      \u2010adrenergic stimulation and resistance training to decipher shared and distinct remodelling patterns.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We collected vastus lateralis biopsies from 21 moderately trained young males (mean\u2009\u00b1\u2009SD, age: 24\u2009\u00b1\u20093) before and after 4\u2010week whole\u2010body resistance training (three sessions\/week) or daily inhalation of beta\n                      <jats:sub>2<\/jats:sub>\n                      \u2010adrenergic agonist terbutaline (4\u2009mg\/day). From each biopsy, we isolated 40 muscle fibres and typified them using myosin\u2010heavy\u2010chain markers. Fibre pools were analysed using LC\u2013MS\/MS\u2010based proteomics.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Beta\n                      <jats:sub>2<\/jats:sub>\n                      \u2010adrenergic stimulation and resistance training both increased peak\u2010power output during bike\u2010ergometer sprinting (+36\u2009W; 95% CI: 11 to 61,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.007 and +27\u2009W; 95% CI: \u22121 to 56,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.062, respectively) with no between\u2010treatments differences (treatment\u2009\u00d7\u2009time interaction:\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.644). Beta\n                      <jats:sub>2<\/jats:sub>\n                      \u2010adrenergic stimulation regulated 15 and 23 proteins in Type I and Type II fibres, respectively, compared to 101 and 65 with resistance training. There was a remarkable fibre type\u2013dependent response, with ~7% of regulated proteins shared between Type I and Type II fibres with resistance training and ~3% with beta\n                      <jats:sub>2<\/jats:sub>\n                      \u2010adrenergic stimulation. Both interventions increased abundance of ribosomal proteins, in which resistance training induced a 25% increase in Type I fibres (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) but only 3% in Type II (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.374), while beta\n                      <jats:sub>2<\/jats:sub>\n                      \u2010adrenergic stimulation increased ribosomal proteins in both fibre types (Type I: 6% increase,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.008; Type II: 9% increase,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Mitochondrial electron\u2010transport\u2010chain protein abundances decreased with both interventions: resistance training reduced abundances mainly in Type I fibres (17% decrease,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001; Type II: 5% decrease,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.147), while beta\n                      <jats:sub>2<\/jats:sub>\n                      \u2010adrenergic stimulation caused uniform decreases (Type I: 7% decrease,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.018; Type II: 9% decrease,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.001). Resistance training uniquely increased contractile, cytoskeletal and extracellular matrix proteins, which was not mimicked by beta\n                      <jats:sub>2<\/jats:sub>\n                      \u2010adrenergic stimulation. S100A13 was upregulated across both interventions and fibre types, whereas MUSTN1 was regulated exclusively with resistance training. Knock\u2010down of S100a13 (\u221252%;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) and Mustn1 (\u221296%;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) in C2C12 myotubes impaired myotube formation (fusion index: S100a13: \u22125%;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.002; Mustn1: \u221221%;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Beta\n                      <jats:sub>2<\/jats:sub>\n                      \u2010adrenergic stimulation induces proteomic adaptations that partially mimic resistance training, particularly in ribosomal proteins. Shared regulation of S100A13 and unique regulation of MUSTN1 with resistance training suggest distinct and complementary roles in regulating muscle growth. These findings indicate that the beta\n                      <jats:sub>2<\/jats:sub>\n                      \u2010adrenergic receptor is a potential target to counter muscle atrophic conditions, offering a pharmacological approach for individuals unable to engage in resistance training.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.70175","type":"journal-article","created":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T07:26:21Z","timestamp":1769412381000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Fibre Type\u2013Specific Proteomics Reveals Shared and Distinct Skeletal Muscle Adaptations to Resistance Training and Beta\n                    <sub>2<\/sub>\n                    \u2010Adrenergic Agonist"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7461-1264","authenticated-orcid":false,"given":"S\u00f8ren","family":"Jessen","sequence":"first","affiliation":[{"name":"Clinical and Experimental Physiology University of Copenhagen  Copenhagen Denmark"},{"name":"The August Krogh Section for Human and Molecular Physiology, Department of Nutrition, Exercise and Sports University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8388-9305","authenticated-orcid":false,"given":"Andrea","family":"Di\u00a0Credico","sequence":"additional","affiliation":[{"name":"Department of Medicine and Aging Sciences University \u201cG. d'Annunzio\u201d of Chieti\u2014Pescara  Chieti 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