{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T15:26:54Z","timestamp":1791473214226,"version":"4.3.4"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:00:00Z","timestamp":1772582400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:00:00Z","timestamp":1772582400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["2024\u2010ER0603\u201001"],"award-info":[{"award-number":["2024\u2010ER0603\u201001"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2024\u201000340798"],"award-info":[{"award-number":["2024\u201000340798"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2021R1A2C2003410"],"award-info":[{"award-number":["2021R1A2C2003410"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS\u20102024\u201000456173"],"award-info":[{"award-number":["RS\u20102024\u201000456173"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS\u20102020\u2010NR049540"],"award-info":[{"award-number":["RS\u20102020\u2010NR049540"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS\u20102023\u201000220894"],"award-info":[{"award-number":["RS\u20102023\u201000220894"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016275","name":"Seoul National University Bundang Hospital","doi-asserted-by":"publisher","award":["18\u20102023\u20100003"],"award-info":[{"award-number":["18\u20102023\u20100003"]}],"id":[{"id":"10.13039\/100016275","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Sarcopenia is an age\u2010related condition characterized by progressive muscle mass, strength and physical performance declines, contributing to frailty and adverse health outcomes. Despite increasing interest in molecular biomarkers, longitudinal data with external validation are limited.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This study applied high\u2010throughput proteomic analysis to identify and validate biomarkers associated with sarcopenia progression in two independent prospective cohorts. The discovery cohort (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009171) was classified into three groups: (1) nonsarcopenic at both baseline and the 2\u2010year follow\u2010up; (2) newly developed sarcopenia; and (3) persistently sarcopenic. The validation cohort (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200993) was followed up for 2\u2009years. Plasma proteomic profiling was conducted using data\u2010independent acquisition (DIA) mass spectrometry. For the validation cohort, targeted quantification (Hyper Reaction Monitoring\u2010DIA) and immunoassays were employed to verify key findings. Statistical analyses included multivariable regression and pathway enrichment analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In the discovery cohort, 102 proteins were differentially expressed between groups (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). Compared to the stable nonsarcopenic group, individuals who developed sarcopenia demonstrated significant APOA1 (fold change \u22121.42,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) and KLKB1 downregulation and LECT2 upregulation. Those who remained sarcopenic exhibited persistent B2M (+1.58,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), S100A9 and LYZ elevation. We identified seven robust protein signatures (LRG1, CST3, TIMP1, C2, ITIH1, AMBP and LYZ) that showed consistent significant associations with sarcopenia components in both cohorts. LRG1 and TIMP1, CST3 and C2 were reproducibly associated with muscle strength, physical performance and muscle mass, respectively. Pathway enrichment analyses consistently highlighted LXR\/RXR signalling, acute phase response signalling and complement cascade activation as central mechanisms across these domains.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study identified and validated plasma protein signatures and pathways associated with sarcopenia progression. Complement activation, acute inflammatory response and lipid dysregulation emerged as central mechanisms. These robustly validated biomarkers may represent targets for early detection and intervention strategies in sarcopenia.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.70240","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T18:37:47Z","timestamp":1772995067000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Distinct Proteomic Signatures Driving Progression of Sarcopenia: A Longitudinal Multicohort Study"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8791-0909","authenticated-orcid":false,"given":"Sung\u00a0Hye","family":"Kong","sequence":"first","affiliation":[{"name":"Department of Internal Medicine Seoul National University Bundang Hospital  Seongnam Republic of Korea"},{"name":"Department of Internal Medicine Seoul National University College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ok\u00a0Hee","family":"Jeon","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Korea University College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u00a0Yeon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Korea University College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miji","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Health Sciences and Technology, College of Medicine Kyung Hee University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhee","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine and Public Health Ajou University School of Medicine  Suwon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung\u00a0Shin","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Seoul National University Hospital  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hak\u00a0Chul","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Seoul National University Bundang Hospital  Seongnam Republic of Korea"},{"name":"Department of Internal Medicine Seoul National University College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang\u00a0Won","family":"Won","sequence":"additional","affiliation":[{"name":"Department of Family Medicine Kyung Hee University Medical Center  Seoul Republic of Korea"},{"name":"Elderly Frailty Research Center, Department of Family Medicine, College of Medicine Kyung Hee University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0841-1598","authenticated-orcid":false,"given":"Dohyun","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Transdisciplinary Medicine Seoul National University Hospital  Seoul Republic of Korea"},{"name":"Department of Medicine Seoul National University College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamda.2019.12.012"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glae237"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02982704"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00154.2020"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2021.101530"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu14071384"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afy169"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-022-01054-z"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.13784"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnha.2025.100607"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2019-035573"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.4235\/agmr.23.0009"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.4235\/agmr.20.0072"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-80120-8"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-35740-1"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3901"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1003"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M100358-JLR200"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41574-018-0037-x"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00061.2018"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11914-021-00656-0"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00561.2011"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.14608"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43587-025-00900-7"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.03.047"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1143578"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-262154"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-017-0354-4"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2005.10.049"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.70109"}],"container-title":["Journal of Cachexia, Sarcopenia and Muscle"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.70240","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcsm.70240","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.70240","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T09:31:14Z","timestamp":1788341474000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcsm.70240"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,4]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/jcsm.70240"],"URL":"https:\/\/doi.org\/10.1002\/jcsm.70240","archive":["Portico"],"relation":{},"ISSN":["2190-5991","2190-6009"],"issn-type":[{"value":"2190-5991","type":"print"},{"value":"2190-6009","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,4]]},"assertion":[{"value":"2025-07-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70240"}}