{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T10:15:40Z","timestamp":1788344140519,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2026,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n The KPC mouse model of pancreatic cancer is gaining use in research, but detailed understanding of ingestive behaviour changes in this and other cachexia models is limited.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Male C57BL\/6\u2009J mice (7\u2009weeks) were maintained under standard feed (PicoLab5L0D) and housing conditions (12:12\u2009h; Light:Dark). Spontaneous feeding was observed in a Comprehensive Lab Animal Monitoring System: meal size, frequency, intermeal intervals and satiety ratio (i.e., time between meals divided by kcal energy in the preceding meal). Feeding was observed at baseline and over time after intraperitoneal injection syngeneic KPC pancreatic adenocarcinoma cells. Separate cohorts of mice were used to study homeostatic feeding responses at baseline and on Day 14 after KPC injection (KPC_14). These responses were fasting\u2010evoked refeeding, ghrelin injection (1\u2009mg\/kg) and lights\u2010out cue.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Spontaneous food intake declined by 50% by 14\u2009days after tumour injection. At baseline, food intake was observed during 23 of 24\u2009h daily; at KPC_14, hours without any food intake increased from 1 to 12 (\n p<\/jats:italic>\n \u2009\u2264\u20090.0001). At KPC_14, intermeal intervals increased (\n light cycle<\/jats:italic>\n : 60.5\u2009\u00b1\u200911.2 vs. 146.4\u2009\u00b1\u200919.6\u2009min;\n p<\/jats:italic>\n \u2009<\u20090.0001; d\n ark cycle<\/jats:italic>\n : 26.0\u2009\u00b1\u20093.8 vs. 42.0\u2009\u00b1\u20095.5\u2009min;\n p<\/jats:italic>\n \u2009=\u20090.0045), and the satiety ratio tripled (\n light cycle<\/jats:italic>\n : 23.1\u2009\u00b1\u20095.9 vs. 69.4\u2009\u00b1\u200910.5\u2009min\/0.1 kcal;\n p<\/jats:italic>\n \u2009<\u20090.0001;\n dark cycle<\/jats:italic>\n : 11.3\u2009\u00b1\u20093.4 vs. 27.1\u2009\u00b1\u20095.0\u2009min\/0.1 kcal;\n p<\/jats:italic>\n \u2009=\u20090.0028). By contrast, meal size and ingestion rate were unchanged. At KPC_14 mice showed reduced responses to homeostatic feeding cues. After 12\u2010h starvation, refeeding intake during 4\u2009h was 0.81\u2009\u00b1\u20090.3\u2009g vs. 0.39\u2009\u00b1\u20090.19\u2009g at KPC_14 (\u221252%,\n p<\/jats:italic>\n \u2009=\u20090.02). Ghrelin response was also blunted 0.66\u2009\u00b1\u20090.19\u2009g vs. 0.42\u2009\u00b1\u20090.27\u2009g at KPC_14 (\u221236%,\n p<\/jats:italic>\n \u2009=\u20090.005). Mice responded to lights\u2010out with ~ 4\u2009h of vigorous feeding, ingesting a total of 0.94\u2009\u00b1\u20090.2\u2009g at baseline; this amount was reduced to 0.55\u2009\u00b1\u20090.3\u2009g (\u221241%,\n p<\/jats:italic>\n \u2009<\u20090.01) at KPC_14, predominantly due to a steep decline in feeding after the end of the first dark hour.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n Mice with KPC show response to homeostatic feeding cues; however, these were of diminished amplitude and sustainability. Large increases in intermeal interval and satiety ratio were notable, with mice eating nothing at all for extended periods of time, consistent with enhanced satiety and\/or a failure to generate signals sufficient to generate a new feeding event.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jcsm.70262","type":"journal-article","created":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T04:40:47Z","timestamp":1775104847000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Feeding Behaviour in the KPC Model of Pancreatic Cancer\u2010Associated Cachexia: Alteration of Spontaneous and Evoked Feeding Behaviours"],"prefix":"10.1002","volume":"17","author":[{"given":"Fateema","family":"Muzaffar","sequence":"first","affiliation":[{"name":"Department of Oncology University of Alberta, Cross Cancer Institute Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William\u00a0F.","family":"Colmers","sequence":"additional","affiliation":[{"name":"Department of Pharmacology University of Alberta Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9609-1001","authenticated-orcid":false,"given":"Vickie\u00a0E.","family":"Baracos","sequence":"additional","affiliation":[{"name":"Department of Oncology University of Alberta, Cross Cancer Institute Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,1]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(10)70218-7"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpph.2"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12225"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2010.06.001"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(02)00710-8"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0026-0495(88)90110-2"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42255-025-01360-4"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aaa5823"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00175.2004"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0246569"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12756"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2023.41.16_suppl.e15195"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI138392"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.13435"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.13466"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42255-022-00606-9"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12596"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3729-14.2015"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4574"},{"issue":"1","key":"e_1_2_6_21_1","first-page":"3","volume":"14","author":"Walton K. 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R.","year":"2007","journal-title":"Journal of Supportive Oncology"}],"container-title":["Journal of Cachexia, Sarcopenia and Muscle"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.70262","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcsm.70262","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.70262","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T09:31:51Z","timestamp":1788341511000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcsm.70262"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":32,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/jcsm.70262"],"URL":"https:\/\/doi.org\/10.1002\/jcsm.70262","archive":["Portico"],"relation":{},"ISSN":["2190-5991","2190-6009"],"issn-type":[{"value":"2190-5991","type":"print"},{"value":"2190-6009","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"2025-08-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70262"}}