{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T10:03:21Z","timestamp":1784887401127,"version":"3.55.0"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2026,6]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n Ageing is an inevitable biological process that contributes to increased prevalence of age\u2010associated diseases, including sarcopenia, defined by progressive loss of muscle mass, functional decline and a heightened risk of injury. Developing effective interventions remains a critical clinical priority. This study employed a natural ageing mouse model to investigate whether noninvasive low\u2010intensity pulsed ultrasound (LIPUS), a therapeutic ultrasound, delivered to the abdomen, could alleviate age\u2010related muscle deterioration and whether its effects were linked to gut microbiota modulation.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n C57BL\/6 mice were maintained until 92\u2009weeks of age, after which abdominal LIPUS stimulation was administered for 8\u2009weeks. At 100\u2009weeks, both forelimb and hind limb grip strength were assessed prior to euthanasia. Faecal samples from the distal colon were collected for microbiota profiling, and gastrocnemius muscles were harvested for downstream analyses.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Naturally aged mice exhibited sarcopenia\u2010like characteristics, including impaired muscle performance, reduced myofiber diameter and decreased muscle weight (\n n<\/jats:italic>\n \u2009=\u20096,\n p<\/jats:italic>\n \u2009<\u20090.01,\n p<\/jats:italic>\n \u2009<\u20090.001). Age\u2010related renal impairment promoted the accumulation of advanced glycation end products (AGEs) in skeletal muscle, triggering pro\u2010inflammatory signalling cascades characterized by elevated COX\u20102, phosphorylated NF\u2010\u03baB, NLRP3, IL\u20101\u03b2 and Caspase\u20101 (\n n<\/jats:italic>\n \u2009=\u20095\u20136,\n p<\/jats:italic>\n \u2009<\u20090.01). LIPUS treatment significantly improved muscle strength (forelimb and hind limb grip strength,\n n<\/jats:italic>\n \u2009=\u20096,\n p<\/jats:italic>\n \u2009<\u20090.001, p\u2009<\u20090.01) and muscle mass (\n n<\/jats:italic>\n \u2009=\u20096,\n p<\/jats:italic>\n \u2009<\u20090.01), while suppressing inflammatory mediators (\n n<\/jats:italic>\n \u2009=\u20095\u20136,\n p<\/jats:italic>\n \u2009<\u20090.05). Gut microbiota analysis showed that LIPUS increased microbial diversity (\n n<\/jats:italic>\n \u2009=\u20095\u20136,\n p<\/jats:italic>\n \u2009<\u20090.05) and altered taxonomic composition, enriching anti\u2010inflammatory taxa such as Lactobacillus, Bifidobacterium, Faecalibaculum and Coriobacteriaceae_UCG_002 (\n n<\/jats:italic>\n \u2009=\u20096, p\u2009<\u20090.05). Correlation analysis indicated that these LIPUS\u2010enriched taxa were positively associated with enhanced muscle performance. These data suggest that LIPUS mitigates sarcopenia in naturally aged mice by restoring muscle integrity and attenuating inflammation, possibly via gut microbiota regulation.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n This study shows that natural ageing in mice induces sarcopenia\u2010like features with inflammatory activation and gut microbiota alterations. Abdominal LIPUS treatment alleviated muscle loss, reduced inflammation and promoted beneficial microbes, rejuvenating the ageing muscle. These findings highlight LIPUS as a safe, noninvasive and potentially translatable strategy for sarcopenia, warranting further investigation of its microbiota\u2013muscle interactions.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jcsm.70291","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T08:11:29Z","timestamp":1776759089000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Gut Microbiota\u2010Linked Benefits of Low\u2010Intensity Pulsed Ultrasound Rejuvenate the Ageing Muscle"],"prefix":"10.1002","volume":"17","author":[{"given":"Jia\u2010Hua","family":"Jhuang","sequence":"first","affiliation":[{"name":"Institute of Toxicology, College of Medicine National Taiwan University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuo\u2010Cheng","family":"Lan","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine Tri\u2010Service General Hospital, National Defense Medical University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting\u2010Yu","family":"Chang","sequence":"additional","affiliation":[{"name":"Institute of Toxicology, College of Medicine National Taiwan University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ding\u2010Cheng","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Geriatrics and Gerontology, College of Medicine and Hospital National Taiwan University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9976-1197","authenticated-orcid":false,"given":"Shing\u2010Hwa","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Toxicology, College of Medicine National Taiwan University Taipei Taiwan"},{"name":"Department of Medical Research China Medical University Hospital, China Medical University Taichung Taiwan"},{"name":"Department of Pediatrics, College of Medicine and Hospital National Taiwan University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,21]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"Economic UNDoI \u201cWorld Population Ageing 2013 \u201d Un2014."},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12147"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3803\/EnM.2020.405"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25084300"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afac220"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2023.2213735"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1109\/TBME.2018.2889669"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.70004"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu9121303"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2024.1418778"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00041.2015"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/msystems.00171\u201016"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/9478630"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00243.2009"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpg.2003.10.008"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10522-019-09837-y"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2006.01.015"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abl9051"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985815625169"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11091418"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12709"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-03917-0"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2013.11.005"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1525795113"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/gly086"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.8.2687-2695.2000"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aan5662"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41574-019-0291-6"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0037971"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2014.07.003"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2010.215665"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10122380"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20240997"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2019.07.001"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12849"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms231810369"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2024.111097"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultras.2023.106984"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.79685"}],"container-title":["Journal of Cachexia, Sarcopenia and Muscle"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.70291","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcsm.70291","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.70291","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T09:18:50Z","timestamp":1784884730000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcsm.70291"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,21]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/jcsm.70291"],"URL":"https:\/\/doi.org\/10.1002\/jcsm.70291","archive":["Portico"],"relation":{},"ISSN":["2190-5991","2190-6009"],"issn-type":[{"value":"2190-5991","type":"print"},{"value":"2190-6009","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,21]]},"assertion":[{"value":"2025-09-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70291"}}