{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T10:33:50Z","timestamp":1793010830339,"version":"4.5.0"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2026,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n Transcriptomic adaptations of ageing skeletal muscle to concurrent resistance training (RT) and high\u2010intensity interval training (HIIT) remain insufficiently characterized. We investigated the effects of a 12\u2010week concurrent RT\u2009+\u2009HIIT intervention on the vastus lateralis transcriptome in middle\u2010aged and older adults and whether polyphenol supplementation modulated these responses.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Thirty\u2010eight adults (55\u201370\u2009years; 17 polyphenol [PP], 21 placebo [PLA]) with paired skeletal muscle RNA\u2010sequencing data participated in a randomized, double\u2010blind trial. Participants completed a 30\u2010day supplementation phase followed by a 12\u2010week supervised RT\u2009+\u2009HIIT programme with continued supplementation. Muscle biopsies were obtained before (PRE) and after (POST) training. Differential expression analyses were adjusted for age, sex and muscle fibre type composition. Differential expression was assessed using FDR\u2009\u2264\u20090.05, with |log\n 2<\/jats:sub>\n FC|\u2009>\u20091 applied as an additional effect\u2010size criterion.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Baseline transcriptomic differences between PP and PLA groups were minimal, with three FDR\u2010significant transcripts, of which only\n HMOX1<\/jats:italic>\n additionally exceeded |log\n 2<\/jats:sub>\n FC|\u2009>\u20091 (FDR\u2009=\u20090.011; log\n 2<\/jats:sub>\n FC\u2009=\u20091.16). The group\u2010by\u2010time interaction analysis identified 14 FDR\u2010significant transcripts, of which eight additionally exceeded |log\n 2<\/jats:sub>\n FC|\u2009>\u20091; these signals were characterized by low or sparse expression and high inter\u2010individual variability, without a coherent supplementation\u2010related transcriptional response. In the pooled paired PRE\u2013POST analysis, 60 transcripts reached FDR significance, of which seven additionally exceeded |log\n 2<\/jats:sub>\n FC|\u2009>\u20091, including upregulation of\n CLLU1<\/jats:italic>\n (log\n 2<\/jats:sub>\n FC\u2009=\u20093.10; FDR\u2009=\u20090.0155),\n PRND<\/jats:italic>\n (log\n 2<\/jats:sub>\n log\n 2<\/jats:sub>\n FC\u2009=\u20092.60; FDR\u2009=\u20090.02) and\n LOX<\/jats:italic>\n , and downregulation of\n MYH1<\/jats:italic>\n (log\n 2<\/jats:sub>\n FC\u2009=\u2009\u22121.3; FDR\u2009=\u20090.0001). Most FDR\u2010significant transcripts showed relatively small estimated effect sizes, and functional enrichment analyses did not identify robust pathway\u2010level adaptations.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n In middle\u2010aged and older adults, 12\u2009weeks of concurrent RT\u2009+\u2009HIIT induced a predominantly low\u2010magnitude skeletal muscle transcriptional response. Selected transcript\u2010level changes were consistent with processes related to extracellular matrix remodelling and contractile regulation; however, the absence of robust pathway\u2010level enrichment limits conclusions regarding coordinated biological pathway adaptations. Polyphenol supplementation did not meaningfully influence the transcriptional response to exercise training.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jcsm.70391","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T10:34:56Z","timestamp":1790332496000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Skeletal Muscle Transcriptomic Responses to Concurrent Training With Polyphenols in Ageing Adults: A Randomized Trial"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2573-4572","authenticated-orcid":false,"given":"A.","family":"Guadalupe\u2010Grau","sequence":"first","affiliation":[{"name":"GENUD Toledo Research Group, Faculty of Sport Sciences University of Castilla\u2010La Mancha Toledo Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red Fragilidad y Envejecimiento Saludable (CIBERFES) Instituto de Salud Carlos III Madrid Spain"},{"name":"Grupo Mixto de Fragilidad y Envejecimiento Exitoso UCLM\u2010SESCAM (TEC2022\u2010007) Instituto de Investigaci\u00f3n Sanitaria de Castilla\u2010La Mancha (IDISCAM), Junta de Comunidades de Castilla\u2010La Mancha (JCCM) Toledo Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Gudra","sequence":"additional","affiliation":[{"name":"Scientific Laboratory of Molecular Genetics Riga Stradi\u0146\u0161 University Riga Latvia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2230-7255","authenticated-orcid":false,"given":"D.","family":"Lismane","sequence":"additional","affiliation":[{"name":"Laboratory of Sports and Nutrition Research Riga Stradi\u0146\u0161 University Riga 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