{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T02:51:44Z","timestamp":1791341504906,"version":"4.3.1"},"reference-count":47,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,12,16]],"date-time":"2017-12-16T00:00:00Z","timestamp":1513382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["0315640C"],"award-info":[{"award-number":["0315640C"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011105","name":"Fourth Framework Programme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011105","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Chemical Tech & Biotech"],"published-print":{"date-parts":[[2018,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n BACKGROUND<\/jats:title>\n Vaccines based on virus\u2010like particles (VLPs) are an alternative to inactivated viral vaccines that combine good safety profiles with strong immunogenicity. In order to be economically competitive, efficient manufacturing is required, in particular downstream processing, which often accounts for major production costs. This study describes the optimization and establishment of a chromatography capturing technique using sulfated cellulose membrane adsorbers (SCMA) for purification of influenza VLPs.<\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n Using a design of experiments approach, the critical factors for SCMA performance were described and optimized. For optimal conditions (membrane ligand density: 15.4\u2009\u00b5mol cm\n \u22122<\/jats:sup>\n , salt concentration of the loading buffer: 24\u2009mmol L\n \u20101<\/jats:sup>\n NaCl, and elution buffer: 920\u2009mmol L\n \u20101<\/jats:sup>\n NaCl, as well as the corresponding flow rates: 0.24 and 1.4\u2009mL min\n \u22121<\/jats:sup>\n ), a yield of 80% in the product fraction was obtained. No loss of VLPs was detected in the flowthrough fraction. Removal of total protein and DNA impurities were higher than 89% and 80%, respectively.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n Use of SCMA represents a significant improvement compared with conventional ion exchanger membrane adsorbers. As the method proposed is easily scalable and reduces the number of steps required compared with conventional purification methods, SCMA could qualify as a generic platform for purification of VLP\u2010based influenza vaccines. \u00a9 2017 The Authors.\n Journal of Chemical Technology & Biotechnology<\/jats:italic>\n published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jctb.5474","type":"journal-article","created":{"date-parts":[[2017,10,10]],"date-time":"2017-10-10T06:32:39Z","timestamp":1507617159000},"page":"1988-1996","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Purification of influenza virus\u2010like particles using sulfated cellulose membrane adsorbers"],"prefix":"10.1002","volume":"93","author":[{"given":"Sofia","family":"B Carvalho","sequence":"first","affiliation":[{"name":"iBET, Instituto de Biologia Experimental e 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