{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T11:18:59Z","timestamp":1783163939463,"version":"3.54.6"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Clinical Ultrasound"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>\n                      Papillary thyroid carcinoma (PTC) is the predominant type of malignant thyroid tumor, with its incidence steadily rising in recent years. Timely diagnosis and intervention are crucial for minimizing metastasis and improving survival rates. This study aims to investigate\n                      <jats:italic>miR\u2010485\u20103p<\/jats:italic>\n                      's diagnostic potential for PTC and its role in malignant progression, offering a novel biomarker for PTC.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This study enrolled 150 benign thyroid nodule (BTN) patients and 200 PTC patients. All participants underwent color Doppler ultrasound (CDUS) to measure relevant parameters (RI, PI, and PSV). Serum\n                      <jats:italic>miR\u2010485\u20103p<\/jats:italic>\n                      levels were quantified using RT\u2010qPCR. ROC analysis was conducted to estimate the diagnostic efficacy of CDUS parameters and\n                      <jats:italic>miR\u2010485\u20103p<\/jats:italic>\n                      . Functional experiments were performed to investigate the role of\n                      <jats:italic>miR\u2010485\u20103p<\/jats:italic>\n                      in PTC cells. Additionally, dual\u2010luciferase reporter assays were utilized to validate the direct targeting relationship between\n                      <jats:italic>miR\u2010485\u20103p<\/jats:italic>\n                      and\n                      <jats:italic>GCNT4<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Elevated CDUS parameters could differentiate between patients with PTC and BTN. Serum\n                      <jats:italic>miR\u2010485\u20103p<\/jats:italic>\n                      levels were markedly enhanced in PTC patients. The combination of\n                      <jats:italic>miR\u2010485\u20103p<\/jats:italic>\n                      and CDUS parameters demonstrated superior diagnostic performance for PTC (AUC\u2009=\u20090.926), with sensitivity and specificity of 0.870 and 0.847. Inhibition of\n                      <jats:italic>miR\u2010485\u20103p<\/jats:italic>\n                      remarkably suppressed the proliferative, migratory, and invasive capacities of PTC cells. Furthermore, mechanistic investigations revealed that\n                      <jats:italic>miR\u2010485\u20103p<\/jats:italic>\n                      directly regulates\n                      <jats:italic>GCNT4<\/jats:italic>\n                      expression, thereby modulating the malignant behavior of PTC cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Preliminary results indicate that the biomarker panel combining serum\n                      <jats:italic>miR\u2010485\u20103p<\/jats:italic>\n                      levels with CDUS parameters shows diagnostic potential for PTC. In\u00a0vitro experiments confirmed the potential of the\n                      <jats:italic>miR\u2010485\u20103p<\/jats:italic>\n                      \/\n                      <jats:italic>GCNT4<\/jats:italic>\n                      axis in modulating the malignant behavior of PTC cells.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcu.70201","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T05:56:07Z","timestamp":1770875767000},"page":"1355-1364","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Serum\n                    <i>miR\u2010485\u20103p<\/i>\n                    Improves Diagnosis of Papillary Thyroid Carcinoma by Color Doppler Ultrasound and Promotes Malignant Behavior of Cancer Cells"],"prefix":"10.1002","volume":"54","author":[{"given":"Tao","family":"Han","sequence":"first","affiliation":[{"name":"Department of Ultrasound General Hospital of Ningxia Medical University  Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Ultrasound General Hospital of Ningxia Medical University  Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinglan","family":"Ke","sequence":"additional","affiliation":[{"name":"Department of Ultrasound General Hospital of Ningxia Medical University  Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Chai","sequence":"additional","affiliation":[{"name":"Department of Ultrasound General Hospital of Ningxia Medical University  Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Ultrasound General Hospital of Ningxia Medical University  Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3878-9305","authenticated-orcid":false,"given":"Guangfei","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Ultrasound General Hospital of Ningxia Medical University  Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-2505-7_2"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmrs.751"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.4183\/aeb.2018.43"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12022\u2010022\u201009707\u20103"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12880\u2010025\u201001615\u20102"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.14670\/HH\u201018\u2010416"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz757"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12325\u2010020\u201001391\u20101"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1530\/EJE\u201019\u20100524"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000037768"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.31857\/S0026898422030077"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/15330338241291637"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/21655979.2021.1995099"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010025\u201002586\u20108"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1182259"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2023.110792"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines12040827"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/kjm2.12799"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420\u2010024\u201002035\u2010x"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12020\u2010022\u201003267\u20106"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549\u2010014\u20102949\u2010z"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.7860\/JCDR\/2016\/18459.8227"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2023.1139362"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2022.100441"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1177\/03936155241303763"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.20452\/pamw.3999"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2024.1337226"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.4103\/jfmpc.jfmpc_981_23"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241310764"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40618\u2010024\u201002467\u20103"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1163309"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.118925"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40291\u2010024\u201000721\u20101"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.1030143"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.12669\/pjms.41.3.10566"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2023.113716"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41746\u2010025\u201001455\u2010y"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.23254"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000040082"}],"container-title":["Journal of Clinical Ultrasound"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcu.70201","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcu.70201","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcu.70201","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T10:46:01Z","timestamp":1783161961000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcu.70201"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,11]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/jcu.70201"],"URL":"https:\/\/doi.org\/10.1002\/jcu.70201","archive":["Portico"],"relation":{},"ISSN":["0091-2751","1097-0096"],"issn-type":[{"value":"0091-2751","type":"print"},{"value":"1097-0096","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,11]]},"assertion":[{"value":"2025-04-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}