{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T12:55:40Z","timestamp":1755694540608,"version":"3.40.5"},"reference-count":29,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,8,19]],"date-time":"2020-08-19T00:00:00Z","timestamp":1597795200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R43 EY028467"],"award-info":[{"award-number":["R43 EY028467"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl J of Devlp Neuroscience"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background\/Objective<\/jats:title><jats:p>Vascular Endothelial Growth Factor (VEGF) is the main driver of angiogenesis during neurodevelopment (i.e., brain and retina). VEGF165 and VEGF121 are the two most prevalent human VEGF isoforms. Although retinopathy of prematurity (ROP), a neuroretinal disorder, is associated with VEGF dysregulation, little is known about the interaction of VEGF isoforms on neuroretinal angiogenesis. We hypothesized that: (a) A specific VEGF165\/VEGF121 correlation, at a given time point, is associated with normal retinal development (no ROP) and (b) An altered correlation, of such, is associated with aberrant retinal development (ROP). Utilizing pre\u2010collected dried blood spots (DBS) from\u00a0&lt;1\u2010week\u2010old preterm infants, we aimed to determine whether correlations between VEGF165 and VEGF121 precede the diagnosis of early stage, non\u2010proliferative ROP (NP\u2010ROP).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>We conducted a case\/control study, utilizing DBS from 65 preterm infants. We measured DBS levels of VEGF165 on the Mesoscale Discovery Platform and VEGF121 via Cloud Clone Elisa Assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In infants with NP\u2010ROP, VEGF165 is significantly higher in males (than females). In infants without ROP, there is a significant correlation between VEGF165 and VEGF121 in females (but not males). In infants with NP\u2010ROP, the opposite is so; there is a significant correlation between VEGF165 and VEGF121 in males (but not females).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This pilot study, utilizing de\u2010identified data, suggests the potential importance of examining interactions between VEGF isoforms, at\u00a0&lt;1\u00a0week after birth, to better understand ROP development. Our study also suggests that retinal angiogenesis may not be a sex\u2010neutral process. A prospective study is needed to confirm our novel findings.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jdn.10054","type":"journal-article","created":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T05:45:05Z","timestamp":1596260705000},"page":"586-593","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Associations between VEGF isoforms and impending retinopathy of prematurity"],"prefix":"10.1002","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1613-7277","authenticated-orcid":false,"given":"Tammy Z.","family":"Movsas","sequence":"first","affiliation":[{"name":"Zietchick Research Institute (ZRI) Plymouth MI USA"},{"name":"Department of Pediatrics and Human Development College of Human Medicine Michigan State University East Lansing MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arivalagan","family":"Muthusamy","sequence":"additional","affiliation":[{"name":"Zietchick Research Institute (ZRI) Plymouth MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,8,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaapos.2014.01.014"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.braindev.2008.09.004"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0887-8994(98)00018-6"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2009.127514"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2003-0027"},{"issue":"1","key":"e_1_2_9_7_1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1167\/iovs.02-1096","article-title":"VEGF and KDR gene expression during human embryonic and fetal eye development","volume":"45","author":"Gogat K.","year":"2004","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"e_1_2_9_8_1","first-page":"e52619","article-title":"Dried blood spots\u2013preparing and processing for use in immunoassays and in molecular techniques","volume":"97","author":"Gruner N.","year":"2015","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2016.09.004"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10633-010-9251-0"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2014.07.050"},{"issue":"12","key":"e_1_2_9_12_1","first-page":"1162","article-title":"Mechanisms and management of retinopathy of prematurity","volume":"368","author":"Hartnett M. 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