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Neuroinflammation influences the blood\u2010brain barrier's (BBB) permeability allowing peripheral immune cell trafficking into the brain. <jats:italic>Mycobacterium tuberculosis<\/jats:italic> (<jats:italic>Mtb<\/jats:italic>) is a pathogen that causes Tuberculosis (TB), a global pandemic responsible for health and economic burdens. Although it is known that maternal infections increase the risk of Autism spectrum disorder (ASD), it is not known whether gestational <jats:italic>Mtb<\/jats:italic> infections also contribute to impaired foetal neurodevelopment. Here we infect pregnant Balb\/c mice with <jats:italic>Mtb<\/jats:italic> H37Rv and Valproic acid (VPA) individually and in combination. Neuroinflammation was measured by assessing microglia and astrocyte population in the prefrontal cortex (PFC) and cerebellum (CER) of pups. <jats:italic>Mtb<\/jats:italic> infection increased the microglia population and caused morphological changes to a reactive phenotype in the PFC. Also, the astrocyte population was significantly increased in the PFC of <jats:italic>Mtb<\/jats:italic> pups. The BBB permeability was determined by measuring the Evans Blue (EB) dye concentration in the PFC and CER 1\u00a0hr post receiving intravenous EB\u2010dye injection. We found that prenatal <jats:italic>Mtb<\/jats:italic> exposure significantly increased the BBB's permeability in the PFC and CER of pups versus saline. Overall, our data demonstrate that prenatal exposure to <jats:italic>Mtb<\/jats:italic> predisposes offspring to a higher risk of BBB damage while inducing persistent neuroinflammation, which could lead to impaired neuronal development and function. These findings implicate a potential role of gestational <jats:italic>Mtb<\/jats:italic> infections in the aetiology of ASD.<\/jats:p>","DOI":"10.1002\/jdn.10116","type":"journal-article","created":{"date-parts":[[2021,5,1]],"date-time":"2021-05-01T09:21:47Z","timestamp":1619860907000},"page":"428-437","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<i>Mycobacterium tuberculosis<\/i> causes a leaky blood\u2010brain barrier and neuroinflammation in the prefrontal cortex and cerebellum regions of infected mice offspring"],"prefix":"10.1002","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4137-7977","authenticated-orcid":false,"given":"Wadzanai","family":"Manjeese","sequence":"first","affiliation":[{"name":"Department of Human Physiology School of Laboratory Medicine and Medical Sciences, College of Health Sciences University of Kwazulu Natal  Durban South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nontobeko E.","family":"Mvubu","sequence":"additional","affiliation":[{"name":"Discipline of Microbiology School of Life Sciences College of Agriculture, Engineering and Science University of Kwazulu Natal  Durban South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrie J. 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