{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:47:19Z","timestamp":1786978039026,"version":"build-2736575974"},"reference-count":27,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T00:00:00Z","timestamp":1659657600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl J of Devlp Neuroscience"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The use of benzodiazepines (BZDs) during pregnancy, especially alprazolam, is common and its impact on the fetal neural tissue is not known. In this sense, the present study aimed to investigate the effects of prenatal treatment with alprazolam on the cerebellum of\n                    <jats:italic>Wistar<\/jats:italic>\n                    rat pups. Thirty animals (24 females and six males, CEUA protocol 014\/17) were separated into pairs for copulation. Females were divided into three groups: Control (CT), treatment 1 (T1, 1.25\u2009mg per animal), and treatment 2, which is an overdose (T2, 30\u2009mg per animal). Alprazolam was administered 10\u2009days before copulation and throughout pregnancy. We evaluated the number and weight of pups and the macroscopic changes in the brain. Eight neonates (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20098) from each group were used in the following analyses: Cellular and chromatin density, gliosis, synaptic density, inflammation, and oxidative stress. The results showed no significant differences regarding the number of pups, body weight, and macroscopic changes. The morphological study focused on the external granular layer (EGL) that is presented only in the immature cerebellum. Here, we detected more cells after alprazolam treatment; the T2 group showed large nuclei and some pyknotic nuclei; also, both treated groups presented an increase in the euchromatin density compared with the control. The molecular and biochemical analyses used the total protein extract of the entire cerebellum and showed an increased expression of Iba\u20101 and NF\u2010\u03baBp65 but without indication of inflammation or degeneration in the T1 group. Overdose of alprazolam presented an increased level of oxidative degradation of lipids. The treatment with alprazolam during pregnancy involved cellular and molecular changes in the immature cerebellum.\n                  <\/jats:p>","DOI":"10.1002\/jdn.10222","type":"journal-article","created":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T06:11:13Z","timestamp":1659420673000},"page":"726-734","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Prenatal effects of alprazolam treatment on the immature cerebellum of rats"],"prefix":"10.1002","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1499-9850","authenticated-orcid":false,"given":"Fernanda Naves Ara\u00fajo do Prado","family":"Mascarenhas","sequence":"first","affiliation":[{"name":"Institute of Biomedical Sciences Federal University of Uberlandia (UFU)  Uberlandia MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6277-0367","authenticated-orcid":false,"given":"Nat\u00e1lia Ferreira","family":"Silva","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences Federal University of Uberlandia (UFU)  Uberlandia MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9913-6255","authenticated-orcid":false,"given":"Lorena Tann\u00fas","family":"Menezes\u2010Reis","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences Federal University of Uberlandia (UFU)  Uberlandia MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3116-3374","authenticated-orcid":false,"given":"Luc\u00e9lia Gon\u00e7alves","family":"Vieira","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences Federal University of Goi\u00e1s (UFG)  Goiania GO Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9438-7538","authenticated-orcid":false,"given":"L\u00edria Queiroz Luz","family":"Hirano","sequence":"additional","affiliation":[{"name":"Faculty of Agronomy and Veterinary Medicine University of Bras\u00edlia (UnB)  Brasilia DF Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9010-3833","authenticated-orcid":false,"given":"Fran\u00e7oise Vasconcelos","family":"Botelho","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology Federal University of Uberlandia (UFU)  Uberlandia MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2800-4084","authenticated-orcid":false,"given":"Daniele Lisboa","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences Federal University of Uberlandia (UFU)  Uberlandia MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5930-3821","authenticated-orcid":false,"given":"Renata Graciele","family":"Zanon","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences Federal University of Uberlandia (UFU)  Uberlandia MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568007033482841"},{"key":"e_1_2_10_3_1","first-page":"235","article-title":"Effects of gamma\u2010aminobutyric acid (GABA) on synaptogenesis and synaptic function","volume":"5","author":"Belhage B.","year":"1998","journal-title":"Perspectives on Developmental Neurobiology"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00017.2006"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1976.9999"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.21815"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(06)60005-8"},{"key":"e_1_2_10_8_1","volume-title":"Avalia\u00e7\u00e3o toxicol\u00f3gica dos benzodiazep\u00ednicos diazepam e maleato de midazolam sobre o desenvolvimento pr\u00e9\u2010natal de ratos Wistar","author":"Gehlen K. 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