{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:09:10Z","timestamp":1790809750446,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,12,17]],"date-time":"2022-12-17T00:00:00Z","timestamp":1671235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003810","name":"Hospital de Cl\u00ednicas de Porto Alegre","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003810","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl J of Devlp Neuroscience"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nowadays, the only treatment for human babies suffering from hypoxia\u2013ischemia (HI) is therapeutic hypothermia (TH). However, a better understanding of the specific effects of TH in males and females is important to improve its clinical application. The present study evaluated the short\u2010term effects of TH on the brain injury and behavioral outcomes in male and female neonatal rats submitted to neonatal HI. Seven\u2010day\u2010old Wistar rats underwent a surgery for unilateral occlusion of the right common carotid artery and were exposed to a hypoxic atmosphere (8% oxygen) for 75\u2009min. Then, the animals in the TH group were submitted to TH (scalp temperature of 32\u00b0C) for 5\u2009h. In the behavioral tests, no remarkable differences triggered by HI or TH were observed relative to SHAM animals. Only females of the HI group presented lower latency to complete the righting reflex test. TH reduced the volume of brain injury in males, but not in females. The animals of the HI group showed a reduction in the number of neurons in the CA1 and dentate gyrus (DG) regions of the hippocampus and TH partially prevented neuronal death. In the CA1 region of the hippocampus, animals from the HI group showed more degenerating cells relative to the SHAM, which was reversed by TH. In the DG, animals from the HI group showed an increase in the number of degenerating neurons, which was partially reversed by TH only in males. Our data show that HI leads to a brain injury, which was attenuated by TH in a sex\u2010dependent way and clarify the importance of the assessment of males and females in order to outline specific strategies for the treatment of each sex in newborns suffering from HI.<\/jats:p>","DOI":"10.1002\/jdn.10244","type":"journal-article","created":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T12:47:06Z","timestamp":1669898826000},"page":"165-177","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Short\u2010term effects of therapeutic hypothermia following hypoxia\u2013ischemia in neonatal male and female rats"],"prefix":"10.1002","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6531-3783","authenticated-orcid":false,"given":"Jana\u00edna","family":"Zang","sequence":"first","affiliation":[{"name":"Postgraduate Program in Biological Sciences: Physiology Federal University of Rio Grande do Sul (UFRGS)  Porto Alegre Brazil"},{"name":"Centro de Pesquisa Experimental Hospital de Cl\u00ednicas de Porto Alegre (HCPA)  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5859-4065","authenticated-orcid":false,"given":"Anna Clara Machado","family":"Colucci","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biological Sciences: Physiology Federal University of Rio Grande do Sul (UFRGS)  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7745-6423","authenticated-orcid":false,"given":"Isadora D'\u00c1vila","family":"Tassinari","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biological Sciences: Physiology Federal University of Rio Grande do Sul (UFRGS)  Porto Alegre Brazil"},{"name":"Centro de Pesquisa Experimental Hospital de Cl\u00ednicas de Porto Alegre (HCPA)  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7450-0233","authenticated-orcid":false,"given":"Ricardo Ribeiro","family":"Nunes","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biological Sciences: Physiology Federal University of Rio Grande do Sul (UFRGS)  Porto Alegre Brazil"},{"name":"Centro de Pesquisa Experimental Hospital de Cl\u00ednicas de Porto Alegre (HCPA)  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6596-3367","authenticated-orcid":false,"given":"Mirella Kielek Galvan","family":"Andrade","sequence":"additional","affiliation":[{"name":"Department of Physiology, Institute of Basic Health Sciences Federal University of Rio Grande do Sul (UFRGS)  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5898-0636","authenticated-orcid":false,"given":"Francielle Fernandes","family":"Spies","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biological Sciences: Physiology Federal University of Rio Grande do Sul (UFRGS)  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8763-1080","authenticated-orcid":false,"given":"Maikel Rosa","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Morphological Sciences, Institute of Basic Health Sciences Federal University of Rio Grande do Sul (UFRGS)  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9035-6275","authenticated-orcid":false,"given":"Luz Elena","family":"Dur\u00e1n\u2010Carabali","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biological Sciences: Physiology Federal University of Rio Grande do Sul (UFRGS)  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0634-9153","authenticated-orcid":false,"given":"Paula","family":"Rigon","sequence":"additional","affiliation":[{"name":"Department of Morphological Sciences, Institute of Basic Health Sciences Federal University of Rio Grande do Sul (UFRGS)  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0596-5667","authenticated-orcid":false,"given":"Carlos Alexandre","family":"Netto","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biological Sciences: Physiology Federal University of Rio Grande do Sul (UFRGS)  Porto Alegre Brazil"},{"name":"Department of Biochemistry, Institute of Basic Health Sciences Federal University of Rio Grande do Sul (UFRGS)  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3668-7054","authenticated-orcid":false,"given":"Ana Helena","family":"Paz","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biological Sciences: Physiology Federal University of Rio Grande do Sul (UFRGS)  Porto Alegre Brazil"},{"name":"Centro de Pesquisa Experimental Hospital de Cl\u00ednicas de Porto Alegre (HCPA)  Porto Alegre Brazil"},{"name":"Department of Morphological Sciences, Institute of Basic Health Sciences Federal University of Rio Grande do Sul (UFRGS)  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7203-3467","authenticated-orcid":false,"given":"Luciano St\u00fcrmer","family":"de Fraga","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biological Sciences: Physiology Federal University of Rio Grande do Sul (UFRGS)  Porto Alegre Brazil"},{"name":"Centro de Pesquisa Experimental Hospital de Cl\u00ednicas de Porto Alegre (HCPA)  Porto Alegre Brazil"},{"name":"Department of Physiology, Institute of Basic Health Sciences Federal University of Rio Grande do Sul (UFRGS)  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12,17]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0247229"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2006.02.131"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1211\/jpp.60.6.0008"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/0963689718766362"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-3472(75)90114-1"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resuscitation.2006.01.017"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.7196\/SAMJ.2020.v110i4.14311"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1037\/1082-989X.7.3.370"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.siny.2021.101256"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.4103\/0972-2327.104323"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-020-01867-9"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.siny.2010.05.005"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2016.00029"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11011-018-0193-7"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-020-00827-0"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3791\/53569"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2019.06.016"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.107.499822"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3109\/00207454.2015.1047016"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD003311.pub3"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2015.00161"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(82)90833-2"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijdevneu.2018.12.002"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.earlhumdev.2010.05.010"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.1702"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706400"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2021.113691"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-015-0251-6"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2020.12.012"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.23828"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1203\/01.PDR.0000113546.03897.FC"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2015.03.005"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(08)61973-1"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000610"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410090206"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.WCB.0000123904.17746.92"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.siny.2021.101257"},{"issue":"6","key":"e_1_2_10_39_1","first-page":"1","article-title":"Xenon combined with therapeutic hypothermia is not neuroprotective after severe hypoxia\u2010ischemia in neonatal rats","volume":"1","author":"Sabir H.","year":"2016","journal-title":"PLoS ONE"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.112.674481"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2011.12.024"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/pr.2017.110"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/2040-7378-2-13"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2013.04.001"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1112066"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2014.10.003"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.5385\/nm.2017.24.4.145"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2020.09.006"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0387-7604(97)00027-2"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.07-2190"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20153809"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep23430"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-67532-2"}],"container-title":["International Journal of Developmental Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jdn.10244","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jdn.10244","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jdn.10244","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T06:08:52Z","timestamp":1692684532000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jdn.10244"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,17]]},"references-count":52,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,4]]}},"alternative-id":["10.1002\/jdn.10244"],"URL":"https:\/\/doi.org\/10.1002\/jdn.10244","archive":["Portico"],"relation":{},"ISSN":["0736-5748","1873-474X"],"issn-type":[{"value":"0736-5748","type":"print"},{"value":"1873-474X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,17]]},"assertion":[{"value":"2022-05-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}