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Folate is crucial for foetal and early postnatal brain development; however, its effects on cerebellar growth and development are unknown. The aim of this study was to examine the effects of maternal folate intake on the histomorphology and cell density of the developing cerebellum. Twelve adult female rats (<jats:italic>rattus norvegicus<\/jats:italic>) were randomly assigned to one of four premixed diet groups: standard (2\u00a0mg\/kg), folate\u2010deficient (0\u00a0mg\/kg), folate\u2010supplemented (8\u00a0mg\/kg) or folate supra\u2010supplemented (40\u2009mg\/kg). The rats started their diets 14\u2009days before mating and consumed them throughout pregnancy and lactation. On postnatal days 1, 7, 21 and 35, five pups from each group were sacrificed, and their brains were processed for light microscopic analysis. Histomorphology and cell density of the external granule, molecular, Purkinje and internal granule layers were obtained. The folate\u2010deficient diet group had smaller, dysmorphic cells and significantly lower densities of external granule, molecular, Purkinje and internal granule cells. Although the folate\u2010enriched groups had greater cell densities than the controls, the folate\u2010supplemented group had considerably higher cell densities than the supra\u2010supplemented group. The folate supra\u2010supplemented group had ectopic Purkinje cells in the internal granule cell layer. These findings imply that a folate\u2010deficient diet impairs cellular growth and reduces cell density in the cerebellar cortex. On the other hand, folate supplementation increases cell densities, but there appears to be an optimal dose of supplementation since excessive folate levels may be detrimental.<\/jats:p>","DOI":"10.1002\/jdn.10337","type":"journal-article","created":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T04:00:25Z","timestamp":1716350425000},"page":"406-422","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Impact of varying maternal dietary folate intake on cerebellar cortex histomorphology and cell density in offspring rats"],"prefix":"10.1002","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5639-2993","authenticated-orcid":false,"given":"Philip Maseghe","family":"Mwachaka","sequence":"first","affiliation":[{"name":"Faculty of Health Sciences University of Nairobi  Nairobi Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Gichangi","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences University of Nairobi  Nairobi Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adel","family":"Abdelmalek","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences University of Nairobi  Nairobi Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Odula","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences University of Nairobi  Nairobi Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julius","family":"Ogeng'o","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences University of Nairobi  Nairobi Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114500000222"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-3806(99)00057-7"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnsys.2021.646052"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2021.e08071"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000147336"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.111.030783"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.63668"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12993-022-00193-5"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.19438"},{"key":"e_1_2_9_11_1","doi-asserted-by":"crossref","unstructured":"Blommers M. 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