{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:01:01Z","timestamp":1760954461641,"version":"build-2065373602"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2002,11,11]],"date-time":"2002-11-11T00:00:00Z","timestamp":1036972800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sea urchins have a set of five continuously growing teeth, each of which has a very complex structure. The mineral phase is calcite of varying Mg content, depending on the location within a tooth. The calcium carbonate is present in amorphous, plate\u2010like and rod\u2010like forms. It has been hypothesized that the mineral deposition is a matrix\u2010mediated process, similar to that in vertebrate bone and tooth, wherein certain macromolecules within the organic matrix of the mineralized tissue play an important role in nucleating and controlling the growth habit of the mineral crystals. It has also been hypothesized that the mineral\u2010related macromolecules involved in urchin teeth might bear a direct evolutionary relationship to those of the vertebrate tooth. These hypotheses are explored here by examining the pattern and nature of the mineral distribution, using microCT of intact teeth, and the nature of the mineral\u2010related matrix proteins. The mineral\u2010related proteins were extracted and fractionated by anion exchange chromatography. The relationship of certain fractions to vertebrate matrix proteins was established by immunoblots using antibodies to vertebrate tooth proteins. The antibodies were then used to localize the proteins within the teeth, by immunocytochemistry and histology with specific staining. The microCT data on mineral density has been correlated with the patterns of cellular migration and mineral deposition within the tooth as it grows. It appears that the mineralization within the different tooth compartments might take place under the influence of different matrix proteins. Further studies are in progress to more completely describe the vertebrate\u2010invertebrate immunologically cross\u2010reactive proteins of the urchin teeth. Microsc. Res. Tech. 59:342\u2013351, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jemt.10216","type":"journal-article","created":{"date-parts":[[2002,11,13]],"date-time":"2002-11-13T08:45:54Z","timestamp":1037177154000},"page":"342-351","source":"Crossref","is-referenced-by-count":29,"title":["Mineral\u2010related proteins of sea urchin teeth: <i>Lytechinus variegatus<\/i>"],"prefix":"10.1002","volume":"59","author":[{"given":"Arthur","family":"Veis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph","family":"Barss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Dahl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed","family":"Rahima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stuart","family":"Stock","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,11,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008209609005269"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004180050290"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215549904700911"},{"key":"e_1_2_1_5_1","first-page":"345","article-title":"Echinoidea","volume":"14","author":"Cavey M","year":"1994","journal-title":"Microsc Anat Invert"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02010765"},{"key":"e_1_2_1_7_1","series-title":"Echinoderm studies","first-page":"109","author":"DuBois P","year":"1989"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(91)90291-A"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1401580304"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(91)90226-S"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.15.9150"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02060296"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7008198"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00298712"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00995664"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00312044"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00312313"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02539801"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02556342"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1130\/0016-7606(1969)80[1613:PPIES]2.0.CO;2"},{"key":"e_1_2_1_21_1","article-title":"Mineral phase microstructure in teeth of the short spined sea urchin (Lytechinus variegatus) studied with X\u2010ray phase contrast imaging and with absorption microtomography","volume":"45","author":"Stock SR","year":"2002","journal-title":"Advances in X\u2010ray Analysis"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1047-8477(02)00500-2"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(87)90254-5"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)43458-8"},{"key":"e_1_2_1_25_1","first-page":"273","volume-title":"Biomineralization and biological metal accumulation","author":"Veis A","year":"1982"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402400106"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1151-2916.1998.tb02444.x"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1997.0034"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1188379"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1999.4096"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1999.4105"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjemt.10216","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.10216","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T16:06:28Z","timestamp":1760630788000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.10216"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,11,11]]},"references-count":30,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2002,12]]}},"alternative-id":["10.1002\/jemt.10216"],"URL":"https:\/\/doi.org\/10.1002\/jemt.10216","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"type":"print","value":"1059-910X"},{"type":"electronic","value":"1097-0029"}],"subject":[],"published":{"date-parts":[[2002,11,11]]}}}