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Angulation always occurs at the site of the cytoplasmic droplet located in mature sperm at the annulus (midpiece \u2013 principal piece junction). This article reviews the literature on the fate of the sperm's cytoplasmic droplet and suggests a role for it in volume regulation. A hypothesis is presented that during epididymal transit sperm acquire osmolytes accumulated by the epididymal epithelium. This is possibly driven by a mechanism of regulatory volume increase invoked by the hypertonicity of epididymal luminal fluid. These osmolytes are subsequently used for regulatory volume decrease after ejaculation into the relatively hypotonic female tract. Study of the mechanisms of sperm volume regulation may highlight new contraceptive leads. Microsc. Res. Tech. 61:28\u201338, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jemt.10314","type":"journal-article","created":{"date-parts":[[2003,3,28]],"date-time":"2003-03-28T07:54:08Z","timestamp":1048838048000},"page":"28-38","source":"Crossref","is-referenced-by-count":165,"title":["Acquisition of volume regulatory response of sperm upon maturation in the epididymis and the role of the cytoplasmic droplet"],"prefix":"10.1002","volume":"61","author":[{"given":"Trevor 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