{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:28:46Z","timestamp":1760660926330,"version":"build-2065373602"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2003,10,29]],"date-time":"2003-10-29T00:00:00Z","timestamp":1067385600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2003,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In order to further our understanding regarding the temporal and topographic ultrastructural aspects of the myocardium under thyrotoxicosis, thyroxine (T4; 25 and 100 \u03bcg\/100 g bw) was administered to young rats 24 hours after birth until 15 days. The animals were then sacrificed, the hearts excised and weighed, and the ventricle tissue samples were then processed for confocal and transmission electron microscopy. At 48\/72 hours and 1 week after initiation of T4 treatment with 100 \u03bcg\/100 g bw, numerous lamellar bodies (probably formed by phospholipids) progressively accumulated in the heart. These bodies were observed in the cytosol, inside mitochondria and in the extracellular matrix. At 2 weeks of T4 treatment with 100 \u03bcg\/100 g bw, lamellar bodies were virtually absent. Changes in cell shape, disorganization of intercellular junctions, and substantial myofibrillar disarray were observed in many cardiomyocytes. A gradient of myofibrillar disarray, which increased in abundance and intensity from the endocardium to the epicardium, was also observed. Immunocytochemical staining for desmin showed that the arrangement of this protein was disorganized in many cells of T4\u2010treated rats as compared with normal ones, confirming ultrastructural data. The predominant appearance of myofibrillar disarray, associated with disorganization of cytoskeletal proteins in the deep myocardium, may be due to higher mechanical wall stress and consequent higher metabolic demand. Alternatively, differential sensitivity of cardiomyocytes to thyroid hormone in different areas is also a possibility. Microsc. Res. Tech. 62:451\u2013459, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jemt.10415","type":"journal-article","created":{"date-parts":[[2003,10,31]],"date-time":"2003-10-31T10:05:30Z","timestamp":1067594730000},"page":"451-459","source":"Crossref","is-referenced-by-count":7,"title":["Temporal and topographic ultrastructural alterations of rat heart myofibrils caused by thyroid hormone"],"prefix":"10.1002","volume":"62","author":[{"given":"Paula Juliana","family":"Ferreira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina","family":"L'Abbate","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Alexandre","family":"Abrahamsohn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cecilia Azevedo","family":"Gouveia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anselmo Sigari","family":"Moriscot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,10,29]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"22","article-title":"Contractile function of the myocardium of the right ventricle in thyrotoxicosis","volume":"21","author":"Abinder AA","year":"1975","journal-title":"Probl Endokrinol (Mosk)"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1247\/csf.22.139"},{"key":"e_1_2_1_4_1","first-page":"4701","article-title":"Endogenous subclinical hyperthyroidism affects quality of life and cardiac morphology and function in young and middle\u2010aged patients","volume":"85","author":"Biondi B","year":"2000","journal-title":"J Clin Endocrinol Metab"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1010056202122"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9149(81)90569-5"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.3109\/13813459209035251"},{"key":"e_1_2_1_8_1","first-page":"26","article-title":"Thyroid hormone induced changes in cardiac proteins and mRNAs","volume":"17","author":"Dillmann WH","year":"1987","journal-title":"Horm Metab Res"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1159\/000176411"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/clc.4960230605"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"3105","DOI":"10.1016\/S0021-9258(17)30188-6","article-title":"Thyroid hormone stimulates synthesis of a cardiac myosin isozyme. 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