{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:29:28Z","timestamp":1790810968426,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4617,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[1992,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Several methods were utilized to visualize the structure and orientation of the blastocoelic extracellular matrix (ECM) in <jats:italic>Strongylocentrotus purpuratus<\/jats:italic> embryos at the mesenchyme blastula stage. Rapid freezing in liquid propane cooled to LN<jats:sub>2<\/jats:sub> temperatures followed by freeze substitution was used to preserve the ECM without shrinkage due to dehydration. Scanning, transmission, and light microscopy were employed to elucidate the ECMs' structure. The blastocoelic ECM consisted of parallel fibrillar sheets that were interconnected by finer filaments and oriented along the animal\u2010vegetal axis. The ECM completely filled the blastocoelic cavity as viewed by scanning electron microscopy. The basal lamina could be distinguished from the blastocoelic ECM as a thin coat on the plasma membrane of epithelial cells; the ECM was in contact with this coat. In contrast, the blastocoelic ECM attached directly to the plasma membrane of primary mesenchyme cells (PMC) which did not possess a basal lamina. The blastocoelic ECM was isolated as an intact \u201cbag\u201d and probed in a hydrated state with Con A and alcian blue. Confocal microscopy confirmed that the entire blastocoel was filled with a fibrillar ECM. These approaches offer advantages for future studies of the ECMs of sea urchin embryos and their roles in gastrulation. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jemt.1070220104","type":"journal-article","created":{"date-parts":[[2005,2,23]],"date-time":"2005-02-23T17:44:53Z","timestamp":1109180693000},"page":"11-22","source":"Crossref","is-referenced-by-count":18,"title":["Preservation and visualization of the sea urchin embryo blastocoelic extracellular matrix"],"prefix":"10.1002","volume":"22","author":[{"given":"Gary N.","family":"Cherr","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John D.","family":"Baldwin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert G.","family":"Summers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John B.","family":"Morrill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"1436","article-title":"Changes in structure and location of extracellular matrix of the blastocoel during development of the starfish Pisaster ochraceus. Can","volume":"64","author":"Abed M.","year":"1986","journal-title":"J. Zool."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-169X.1989.00131.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-1605(88)80932-7"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-169X.1990.00353.x"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00848108"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00375747"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051850310"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.1060030206"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00848500"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(80)80019-0"},{"key":"e_1_2_1_12_1","first-page":"1","volume-title":"The Role of Extracellular Matrix in Development","author":"Hay E. D.","year":"1984"},{"key":"e_1_2_1_13_1","first-page":"277","volume-title":"Extracellular Matrix Biochemistry","author":"Heinegard D.","year":"1984"},{"key":"e_1_2_1_14_1","first-page":"305","article-title":"2,2\u2010dimethoxypropane, a rapid dehydrating agent for scanning electron microscopy","volume":"7","author":"Johnson W. S.","year":"1976","journal-title":"Micron"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402100320"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(81)90281-5"},{"key":"e_1_2_1_17_1","first-page":"25","article-title":"Role of the extracellular matrix in amphibian gastralation","volume":"1","author":"Keller R.","year":"1990","journal-title":"Semin. Dev. Biol."},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091710303"},{"key":"e_1_2_1_19_1","series-title":"45th Symposium Soc. Dev. Biol.","first-page":"111","volume-title":"Genetic Regulation of Development","author":"McClay D.","year":"1987"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.03.110187.001535"},{"issue":"28","key":"e_1_2_1_21_1","first-page":"463","article-title":"On the formation of the blastocoele and similar embryonic cavities","volume":"1","author":"Monne L.","year":"1951","journal-title":"Ark. Zool."},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(50)90024-3"},{"key":"e_1_2_1_23_1","first-page":"3","volume-title":"The Cellular and Molecular Biology of Invertebrate Development","author":"Morrill J. B.","year":"1985"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(08)60353-2"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01417946"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/icb\/24.3.615"},{"key":"e_1_2_1_27_1","first-page":"464","article-title":"Demonstration of the mucous layer of the tear film by electron microscopy","volume":"26","author":"Nichols B. A.","year":"1985","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"e_1_2_1_28_1","first-page":"672","volume-title":"Histochemistry: Theoretical and Applied","author":"Pearse A. G. E.","year":"1968"},{"key":"e_1_2_1_29_1","first-page":"2","volume-title":"Principles and Techniques of Electron Microscopy: Biological Applications","author":"Rebhun L. I.","year":"1974"},{"key":"e_1_2_1_30_1","first-page":"391","volume-title":"Developmental Biology: A comprehensive synthesis","author":"Solursh M.","year":"1986"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(83)90440-8"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051990107"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.1978.tb00120.x"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-169X.1972.00063.x"},{"key":"e_1_2_1_35_1","first-page":"85a","article-title":"Optimal preservation of sea urchin blastocoelic matrix by freeze\u2010substitution fixation","volume":"105","author":"Summers R. G.","year":"1987","journal-title":"J. Cell Biol."},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1120100205"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(90)90087-Q"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(63)90300-8"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00214003"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjemt.1070220104","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.1070220104","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T19:21:16Z","timestamp":1760642476000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.1070220104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,6,15]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,6,15]]}},"alternative-id":["10.1002\/jemt.1070220104"],"URL":"https:\/\/doi.org\/10.1002\/jemt.1070220104","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"value":"1059-910X","type":"print"},{"value":"1097-0029","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,6,15]]}}}