{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T02:47:12Z","timestamp":1774666032547,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4083,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[1993,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to describe and compare the ultrastructural features and functional maturity of alveolar epithelial cells in hypoplastic and normal fetal rat lungs. Pulmonary hypoplasia in association with congenital diaphragmatic hernia was induced in fetuses by administration of 2,4\u2010dichlorophenyl\u2010p\u2010nitrophenylether (Nitrofen) to pregnant Sprague Dawley rats (100 mg on day 10 of gestation). Lung tissue of Nitrofen\u2010exposed and control fetal rats aged 19\u201322 days (vaginal plug day 1, birth day 23) was embedded in Epon. Semithin (1 \u03bcm) toluidine blue\u2010stained sections were examined by light microscopy; ultrathin sections (\u223c80 nm) were studied via transmission electron microscopy. In bronchoalveolar lavage fluid from control and Nitrofenexposed fetuses (day 22), phospholipid fractions and surfactant protein A content were measured semiquantitatively. On day 19 both control and Nitrofen\u2010exposed lungs contained only cuboid alveolar epithelial cells; from day 20 there were cuboid, low cuboid, and thinner epithelial cells. The (low) cuboid cells contained large glycogen fields, some precursory stages of multilamellar bodies (MLBs), and just a few mature MLBs on day 19 and 20; smaller glycogen fields, more precursory stages, and more mature MLBs on day 21; and little or no glycogen but many precursory stages and mature MLBs on day 22. The thinner cells contained little or no glycogen and a few precursory stages of MLBs on days 20\u201322; very thin cells on day 22 contained neither glycogen nor any precursory stages of MLBs. MLBs and tubular myelin were seen in the lumens of future air spaces from day 20 onward. Nitrofen\u2010exposed lungs differed from control lungs in that inclusion bodies (IBs) were less numerous in (low) cuboid alveolar cells on days 19 and 20, and more glycogen was seen on day 22. In addition intra\u2010 and extracellular \u201cMLBs\u201d in exposed lungs more often had an unusual appearance, i.e., a confluent structure and higher electron density. However, despite morphologic differences, there was no clear difference in phospholipid composition and SP\u2010A content per mol phospholipid in bronchoalveolar lavage fluid. We conclude that morphologically hypoplastic lungs are less mature near term, without an apparent effect on surfactant composition. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jemt.1070260507","type":"journal-article","created":{"date-parts":[[2005,2,23]],"date-time":"2005-02-23T17:21:27Z","timestamp":1109179287000},"page":"389-399","source":"Crossref","is-referenced-by-count":20,"title":["Ultrastructural features of alveolar epithelial cells in the late fetal pulmonary acinus: A comparison between normal and hypoplastic lungs using a rat model of pulmonary hypoplasia and congenital diaphragmatic hernia"],"prefix":"10.1002","volume":"26","author":[{"given":"Annelies E.","family":"Brandsma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dick","family":"Tibboel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irma M.","family":"Vulto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johannes","family":"Egberts","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ank A. W.","family":"Ten Have\u2010Opbroek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"736","article-title":"Derivation of type 1 epithelium from type 2 cells in the developing rat lung","volume":"32","author":"Adamson I. Y. R.","year":"1975","journal-title":"Lab. Invest."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.54.8.614"},{"key":"e_1_2_1_4_1","first-page":"262L","article-title":"Surfactant phospholipids: synthesis and storage","volume":"262","author":"Batenburg J. J.","year":"1992","journal-title":"Am. J. Physiol."},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.1982.tb03626.x"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.1950030509"},{"issue":"275","key":"e_1_2_1_7_1","article-title":"Congenital diaphragmatic hernia: studies of lung composition and structure","volume":"115","author":"Blackburn W. R.","year":"1977","journal-title":"Am. Rev. Respir. Dis."},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)92151-Q"},{"key":"e_1_2_1_9_1","first-page":"A126","article-title":"Alveolar epithelial composition and architecture in a rat model of lung hypoplasia","volume":"145","author":"Brandsma A. E.","year":"1992","journal-title":"Am. Rev. Respir. Dis."},{"key":"e_1_2_1_10_1","first-page":"A131","article-title":"Type I cell differentiation and surfactant protein SP\u2010A reactivity in fetal rat lung","volume":"145","author":"Brandsma A. E.","year":"1992","journal-title":"Am. Rev. Respir. Dis."},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.3109\/01902148509087809"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.3109\/01902149209031685"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/S0021-9258(18)84756-1","article-title":"The colorimetric determination of phosphorus","volume":"64","author":"Fiske C. H.","year":"1925","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_14_1","first-page":"A308","article-title":"Pathophysiology of congenital diaphragmatic hernia. III: Exogenous Surfactant Therapy (EST) for the high risk neonate with CDH","volume":"143","author":"Glick P. L.","year":"1991","journal-title":"Am. Rev. Respir. Dis."},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-3468(92)90865-5"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3468(85)80218-9"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(84)90614-8"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/tera.1420260211"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091630405"},{"key":"e_1_2_1_20_1","first-page":"456","article-title":"The effect of 2,4\u2010dichlorophenyl\u2010p\u2010nitrophenyl ether on the rat fetus","volume":"25","author":"Kimbrough R. D.","year":"1973","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/00039896.1974.10666499"},{"key":"e_1_2_1_22_1","first-page":"67","article-title":"Fetal lung development","volume":"6","author":"Kitterman J. A.","year":"1984","journal-title":"J. Dev. Physiol."},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-3468(90)90190-K"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(88)90359-6"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(06)80131-9"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.8670137"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-3468(91)90497-H"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/137.1.167"},{"key":"e_1_2_1_29_1","first-page":"372","article-title":"Pulmonary acinar development in diaphragmatic hernia","volume":"115","author":"Nakamura Y.","year":"1991","journal-title":"Arch. Pathol. Lab. Med."},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb\/3.4.325"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00309849"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00305011"},{"key":"e_1_2_1_33_1","series-title":"Mod. Probl. Paediatr","first-page":"28","volume-title":"Congenital Diaphragmatic Hernia","author":"Pringle K. C.","year":"1989"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0300-483X(81)90051-2"},{"key":"e_1_2_1_35_1","first-page":"36","volume-title":"American Pediatric Surgical Association","author":"Suen H. C.","year":"1992"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-3468(90)90386-N"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(75)90115-3"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(79)90301-4"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001620303"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.3109\/01902149109064406"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092210409"},{"key":"e_1_2_1_42_1","first-page":"317","article-title":"Ultrastructural charcteristics of inclusion bodies of type II cells in late embryonic mouse lung","volume":"181","author":"Ten Have\u2010Opbroek A. A. W.","year":"1990","journal-title":"Anat. Embryol."},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092340111"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02534120"},{"key":"e_1_2_1_45_1","first-page":"63","volume-title":"Proceedings of the 7th Int. Workshop Surfactant Replacement","author":"Tovar J. A.","year":"1992"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(91)90425-Q"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.56.8.606"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb\/5.1.41"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjemt.1070260507","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.1070260507","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T14:13:42Z","timestamp":1760624022000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.1070260507"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,12]]},"references-count":47,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1993,12]]}},"alternative-id":["10.1002\/jemt.1070260507"],"URL":"https:\/\/doi.org\/10.1002\/jemt.1070260507","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"value":"1059-910X","type":"print"},{"value":"1097-0029","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,12]]}}}