{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T15:41:29Z","timestamp":1784043689649,"version":"3.55.0"},"reference-count":58,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2001,6,5]],"date-time":"2001-06-05T00:00:00Z","timestamp":991699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2001,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The quality of ulcer repair remains crucial for the stability of the injured tissue and for preventing recurrence. Therefore, we studied the temporo\u2010spatial expression of the fibrillar and basement membrane collagens (types I, III, and IV), the collagenase MMP\u20102 as well as its inhibitor TIMP\u20101 before and after oral administration of basic fibroblast growth factor (b\u2010FGF) over 30 days in acetic acid\u2010induced rat gastric ulcers. The alterations and the exact location of the mRNA transcripts and their precipitated proteins were visualized by means of radioactive in situ hybridization and immunohistochemistry. Our data show that hybridization signals of procollagen I could first be identified 2 hours after ulcer induction. After 12 hours the ulcer was established and the mRNA was enhanced at the ulcer margin. After 24\u201348 hours the other procollagen transcripts were detected and all were further upregulated over the mesenchymal cells of all gastric layers up to 21 days, then declined at 30 days. In contrast, MMP\u20102 became prominent after 48 hours and up to 21 days. TIMP\u20101 was enhanced at 72 hours. After oral administration of b\u2010FGF the transcriptional activity of the procollagens and MMP\u20102 was not significantly altered, while ulcer diameter was significantly reduced. We conclude that the early onset and long duration of collagens' expression points to their central structural and functional role in gastric ulcer healing. MMP\u20102 seems to be involved in both active ulceration and ECM remodeling. The timing of TIMP\/MMP expression may be critical for proper restoration of gastric wall integrity. Microsc. Res. Tech. 53:396\u2013408, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jemt.1108","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T17:26:02Z","timestamp":1030296362000},"page":"396-408","source":"Crossref","is-referenced-by-count":45,"title":["Remodeling of extracellular matrix in gastric 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