{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:33:30Z","timestamp":1760661210238,"version":"build-2065373602"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,12,16]],"date-time":"2004-12-16T00:00:00Z","timestamp":1103155200000},"content-version":"vor","delay-in-days":76,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fibrosis and nodular regeneration are the hallmarks of liver cirrhosis. To assess the degree of fibrosis and the severity of the structural changes affecting parenchymal and extraparenchymal components in liver cirrhosis, a computerized morphometric model has been applied to liver specimens from patients undergoing liver transplantation for primary biliary cirrhosis, posthepatitic and alcoholic cirrhosis. Fifty\u2010eight hepatectomy specimens from patients undergoing liver transplantation for cirrhosis were analyzed: 17 alcoholic, 28 posthepatitic (HBV\u2010related and HCV\u2010related cirrhosis), and 13 primary biliary cirrhoses. Liver specimens were fixed in 10% neutral\u2010buffered formalin and embedded in paraffin. Sections were stained with chromotrope\u2010aniline blue method and monoclonal antibodies against cytokeratin 7 and CD31. Volume fractions of parenchymal compartment and fibrosis were stereologically determined on the specimens stained with chromotrope\u2010aniline blue method. Volume fractions of portal bile ducts, proliferated bile ductules, and hepatocytes with biliary metaplasia were measured on cytokeratin 7 stains, while volume fractions of capillary units have been evaluated on CD31 staining. Volume fraction of fibrosis was higher in primary biliary cirrhosis than in the other disease\u2010induced cirrhosis. The main differences were related to immunohistochemical staining. Volume fraction of hepatocytes with biliary metaplasia was higher in HCV\u2010related cirrhosis, whereas volume fractions of biliary structures were more prominent in HBV\u2010related cirrhosis. Primary biliary cirrhosis was characterized by a reduced number of bile ducts and by a wider expression of cytokeratin 7 into periportal hepatocytes. Capillary units were more prominent in primary biliary cirrhosis than alcoholic and posthepatitic cirrhosis. Our computerized morphometric model well describes and quantifies the morphological alterations of the liver and it could represent an adjunctive tool to evaluate the degree of dysplastic phenomena involving parenchymal and extraparenchymal compartments. Microsc. Res. Tech. 65:113\u2013121, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jemt.20110","type":"journal-article","created":{"date-parts":[[2004,12,16]],"date-time":"2004-12-16T12:47:55Z","timestamp":1103201275000},"page":"113-121","source":"Crossref","is-referenced-by-count":12,"title":["Computerized morphometry of the cirrhotic liver: Comparative analysis in primary biliary cirrhosis, alcoholic cirrhosis, and posthepatitic cirrhosis"],"prefix":"10.1002","volume":"65","author":[{"given":"Maurizio","family":"Vertemati","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ernesto","family":"Minola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Goffredi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guido","family":"Sabatella","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcello","family":"Gambacorta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Vizzotto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,12,16]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"521","article-title":"The morphology of cirrhosis: definition, nomenclature and classification","volume":"55","author":"Anthony PP","year":"1977","journal-title":"Bull World Health Org"},{"key":"e_1_2_1_3_1","first-page":"161","article-title":"Pathogenesis of hepatocellular carcinoma: sequential cellular, molecular and metabolic changes","volume":"14","author":"Bannasch P","year":"1996","journal-title":"Prog Liver Dis"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.54.2.148"},{"key":"e_1_2_1_5_1","first-page":"505","article-title":"A rapid Mallory trichrome stain (chromotrope\u2010aniline blue)","volume":"62","author":"Churg J","year":"1956","journal-title":"Arch Pathol"},{"volume-title":"Quantitative microscopy","year":"1968","author":"De Hoff RT","key":"e_1_2_1_6_1"},{"key":"e_1_2_1_7_1","first-page":"544","article-title":"Proc\u00e9d\u00e9 m\u00e9chanique pour d\u00e9terminer la composition des roches","volume":"51","author":"Delesse A","year":"1847","journal-title":"CR Acad Sci D"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1971.51.1.158"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1128\/jcm.30.8.2145-2149.1992","article-title":"Use of aminotransferase, hepatitis C antibody, and hepatitis C polymerase chain reaction RNA assays to establish the diagnosis of hepatitis C virus infection in a diagnostic virology laboratory","volume":"30","author":"Gretch D","year":"1992","journal-title":"J Clin Microbiol"},{"key":"e_1_2_1_10_1","first-page":"285","article-title":"Differential diagnosis of hepatocellular nodular lesions","volume":"15","author":"Hytiroglou P","year":"1998","journal-title":"Semin Diagn Pathol"},{"issue":"3","key":"e_1_2_1_11_1","first-page":"151","article-title":"Application of keratin immunocytochemistry and sirius red staining in evaluating intrahepatic changes with acute extrahepatic cholestasis due to hepatic duct carcinoma","volume":"36","author":"James J","year":"1989","journal-title":"Hepatogastroenterology"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510250439"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199611213352107"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)15108-2"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2008-1040584"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2002.tb04090.x"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004836-200211002-00003"},{"key":"e_1_2_1_18_1","first-page":"126","volume-title":"A manual of morphometry in diagnostic pathology","author":"Oberholzer M","year":"1983"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-199806000-00002"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(99)00149-4"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-1007128"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1970.tb37005.x"},{"key":"e_1_2_1_23_1","first-page":"228","article-title":"Pathological aspects of cirrhosis","volume":"87","author":"Popper H","year":"1977","journal-title":"Am J Pathol"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)15109-4"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1827.1996.tb03609.x"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0676.1993.tb00603.x"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510300507"},{"key":"e_1_2_1_28_1","first-page":"387","article-title":"Primary biliary cirrhosis: chronic non\u2010suppurative destructive cholangitis","volume":"46","author":"Rubin E","year":"1965","journal-title":"Am J Pathol"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.53.10.765"},{"key":"e_1_2_1_30_1","first-page":"382","article-title":"Morphometry of liver parenchyma in needle biopsy specimens from patients with alcoholic liver disease: preliminary variables for diagnosis and prognosis of cirrhosis","volume":"9","author":"Ryoo JW","year":"1980","journal-title":"Mod Pathol"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1177\/003591576706001205"},{"key":"e_1_2_1_32_1","first-page":"173","volume-title":"Liver biopsy interpretation","author":"Scheuer PJ","year":"2000"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0344-0338(11)80727-2"},{"key":"e_1_2_1_34_1","first-page":"357","volume-title":"Diseases of the liver and biliary system","author":"Sherlock S","year":"1992"},{"key":"e_1_2_1_35_1","first-page":"433","article-title":"Ductular hepatocytes","volume":"10","author":"Sirica AE","year":"1995","journal-title":"Histol Histopathol"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(90)91030-A"},{"volume-title":"Statistical methods","year":"1972","author":"Snedecor GW","key":"e_1_2_1_37_1"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(84)90295-2"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1989.tb03060.x"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001850407"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.2001.19850603.x"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.MP.0000024365.92937.5E"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.42.1.68"},{"key":"e_1_2_1_44_1","first-page":"63","volume-title":"Stereological Methods","author":"Weibel ER","year":"1979"},{"key":"e_1_2_1_45_1","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1016\/S0016-5085(82)80006-1","article-title":"Perivenular fibrosis in alcoholic liver injury: ultrastructure and histologic progression","volume":"83","author":"Worner TM","year":"1982","journal-title":"Gastroenterology"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510230607"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjemt.20110","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.20110","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T18:21:18Z","timestamp":1760638878000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.20110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,10]]},"references-count":45,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,10]]}},"alternative-id":["10.1002\/jemt.20110"],"URL":"https:\/\/doi.org\/10.1002\/jemt.20110","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"type":"print","value":"1059-910X"},{"type":"electronic","value":"1097-0029"}],"subject":[],"published":{"date-parts":[[2004,10]]}}}