{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:24:59Z","timestamp":1760660699407,"version":"build-2065373602"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,2,2]],"date-time":"2006-02-02T00:00:00Z","timestamp":1138838400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to assess the dynamics of osteoclast migration and the degradation of unmineralized extracellular matrix in an osteolytic metastasis by examining a well\u2010standardized lung cancer metastasis model of nude mice. SBC\u20105 human lung small carcinoma cells were injected into the left cardiac ventricle of 6\u2010week\u2010old BALB\/c nu\/nu mice under anesthesia. At 25\u201330 days after injection, the animals were sacrificed and their femora and\/or tibiae were removed for histochemical analyses. Metastatic lesions were shown to occupy a considerable area extending from the metaphyses to the bone marrow region. Tartrate resistant acid phosphatase (TRAPase)\u2010positive osteoclasts were found in association with an alkaline phosphatase (ALPase)\u2010positive osteoblastic layer lining the bone surface, but could also be localized in the ALPase\u2010negative stromal tissues that border the tumor nodules. These stromal tissues were markedly positive for osteopontin, and contained a significant number of TRAPase\u2010positive osteoclasts expressing immunoreactivity for CD44. We thus speculated that, mediating its affinity for CD44, osteopontin may serve to facilitate osteoclastic migration after their formation associated with ALPase\u2010positive osteoblasts. We next examined the localization of cathepsin K and matrix metallo\u2010proteinase\u20109 (MMP\u20109) in osteoclasts. Osteoclasts adjacent to the bone surfaces were positive for both proteins, whereas those in the stromal tissues in the tumor nests showed only MMP\u20109 immunoreactivity. Immunoelectron microscopy disclosed the presence of MMP\u20109 in the Golgi apparatus and in vesicular structures at the baso\u2010lateral cytoplasmic region of the osteoclasts found in the stromal tissue. MMP\u20109\u2010positive vesicular structures also contained fragmented extracellular materials. Thus, osteoclasts appear to either select an optimized function, namely secreting proteolytic enzymes from ruffled borders during bone resorption, or recognize the surrounding extracellular matrix by mediating osteopontin\/CD44 interaction, and internalize the extracellular matrices. Microsc. Res. Tech. 69:73\u201383, 2006. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jemt.20272","type":"journal-article","created":{"date-parts":[[2006,2,2]],"date-time":"2006-02-02T13:47:49Z","timestamp":1138888069000},"page":"73-83","source":"Crossref","is-referenced-by-count":20,"title":["Histochemical evidence of osteoclastic degradation of extracellular matrix in osteolytic metastasis originating from human lung small carcinoma (SBC\u20105) cells"],"prefix":"10.1002","volume":"69","author":[{"given":"Minqi","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norio","family":"Amizuka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiichi","family":"Takeuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo 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