{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T09:08:19Z","timestamp":1769850499501,"version":"3.49.0"},"reference-count":29,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2006,8,18]],"date-time":"2006-08-18T00:00:00Z","timestamp":1155859200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The cyst of <jats:italic>Artemia<\/jats:italic> has shell and membranous coverings over the embryo. The membranous coverings have special adaptive features to allow the physical changes accompanying repeated hydration and dehydration cycles that might occur and adversely influence postembryonic development. Whole and slices of cryptobiotic cysts were processed for electron microscopy to study the internal details and to compare the morphological architecture of three <jats:italic>Artemia<\/jats:italic> strains of South India. Surface topography of scanning electron microscopic (SEM) studies revealed distinct button shaped structures on the cyst of Puthalam strain. Transmission electron microscopic (TEM) studies of the cysts displayed the conventional pattern of anostracan crustaceans with outer cortex and alveolar layer, cuticular membranes, and the cytoplasmic inclusions namely nucleus, yolk droplets, lipoid bodies, and mitochondria. The prominent wavy outer cortex layer of Puthalam cysts corroborates the results of SEM studies. Microsc. Res. Tech. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jemt.20371","type":"journal-article","created":{"date-parts":[[2006,8,18]],"date-time":"2006-08-18T20:46:31Z","timestamp":1155933991000},"page":"957-963","source":"Crossref","is-referenced-by-count":25,"title":["Ultrastructure of cyst shell and underlying membranes of three strains of the brine shrimp <i>Artemia<\/i> (Branchiopoda: Anostraca) from South India"],"prefix":"10.1002","volume":"69","author":[{"given":"V.","family":"Sugumar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Munuswamy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,8,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(70)80025-9"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.2307\/1549083"},{"key":"e_1_2_1_4_1","first-page":"313","article-title":"Egg morphology in African Streptocephalidae (Crustacea: Branchiopoda: Anostraca) I: South of Zambezi and Kunene rivers","volume":"99","author":"Brendonck L","year":"1994","journal-title":"Arch Hydrobiol"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.2307\/1937408"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1096-4959(01)00300-1"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.1979.tb00995.x"},{"key":"e_1_2_1_8_1","first-page":"998","article-title":"Resistance des ocufs durables hydrates d'Artemia salina d' \u00e0 l'anoxic","volume":"263","author":"Dutrieu J","year":"1966","journal-title":"CR Hebd Seanc Acad Sci Paris D"},{"key":"e_1_2_1_9_1","first-page":"296","article-title":"Branchinecta sandiegoensis, a new species of fairy shrimp (Crustacea: Anostraca) from Western North America","volume":"106","author":"Fugate M","year":"1993","journal-title":"Proc Biol Soc Wash"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00209045"},{"key":"e_1_2_1_11_1","first-page":"573","volume-title":"Developmental biology of the sea urchin embryos","author":"Guidice G","year":"1973"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402530308"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1959.0013"},{"key":"e_1_2_1_14_1","unstructured":"1994 San Francisco Press San Francisco KW Lee MA Gothro D Belk JR Rosowski GW Bailey AJ Garratti\u2010Reed Ultrastructure features of the tertiary envelope in the cyst of the brine shrimp Artemia franciscana (Anostraca) 362"},{"key":"e_1_2_1_15_1","first-page":"75","article-title":"The egg shells of the brine shrimp, Artemia","volume":"78","author":"Lochead JH","year":"1940","journal-title":"Anat Rec"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(67)90285-7"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.2307\/1548468"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0248-4900(88)90127-X"},{"key":"e_1_2_1_19_1","first-page":"233","article-title":"Ultrastructure of the cyst shell and underlying membranes of the brine shrimp Artemia franciscana Kellogg (Anostraca) during postencystic development, emergence and hatching","volume":"16","author":"Rosowski JR","year":"1997","journal-title":"J Shellfish Res"},{"key":"e_1_2_1_20_1","first-page":"102","article-title":"Excystment of the egg of Artemia salina in artificial seawater of various conditions","volume":"15","author":"Sato NL","year":"1967","journal-title":"Gunma J Med Sci"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.58.3.643"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1014591503151"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00026008"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402430208"},{"key":"e_1_2_1_25_1","first-page":"113","article-title":"Mitochondrial maturation during Artemia salina embryogenesis. 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