{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:22:47Z","timestamp":1760660567433,"version":"build-2065373602"},"reference-count":18,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2006,8,29]],"date-time":"2006-08-29T00:00:00Z","timestamp":1156809600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Centrosomes are small organelles that organize the mitotic spindle during cell division and are also involved in cell shape and polarity. Within epithelial tumors, such as breast cancer, and some hematological tumors, centrosome abnormalities (CAs) are common, occur early in disease etiology, and correlate with chromosomal instability and disease stage. In situ quantification of CA by optical microscopy is hampered by overlap and clustering of these organelles, which appear as focal structures. CA has been frequently associated with <jats:italic>Tp53<\/jats:italic> status in premalignant lesions and tumors. Here the authors described an approach to accurately quantify centrosome frequencies in tissue sections and tumors, independently of background or noise levels. Applying simple optical rules in nondeconvolved conventional 3D images of stained tissue sections, the authors showed that they could evaluate more accurately and rapidly centrosome frequencies than with traditional investigator\u2010based visual analysis or threshold\u2010based techniques. The resulting population\u2010based frequency of centrosomes per nucleus could then be used to approximate the proportion of cells with CA in that same population. This was done by taking into account baseline centrosome amplification and proliferation rates measured in the tissue. Using this technique, the authors showed that 20\u201330% of cells have amplified centrosomes in <jats:italic>Tp53<\/jats:italic> null mammary tumors. Microsc. Res. Tech., 2006. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jemt.20372","type":"journal-article","created":{"date-parts":[[2006,8,29]],"date-time":"2006-08-29T18:49:55Z","timestamp":1156877395000},"page":"964-972","source":"Crossref","is-referenced-by-count":5,"title":["Intensity\u2010based signal separation algorithm for accurate quantification of clustered centrosomes in tissue sections"],"prefix":"10.1002","volume":"69","author":[{"given":"Markus C.","family":"Fleisch","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher A.","family":"Maxwell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia K.","family":"Kuper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erika T.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary Helen","family":"Barcellos\u2010Hoff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvain V.","family":"Costes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,8,29]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.2000.4916"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-09-3810"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600393"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10911-004-1405-9"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.271.5256.1744"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1201482"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.00-0165com"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1905840"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0408273101"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.032479999"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1158\/0008-5472.850.65.3","article-title":"Receptor for hyaluronan\u2010mediated motility correlates with centrosome abnormalities in multiple myeloma and maintains mitotic integrity","volume":"65","author":"Maxwell CA","year":"2005","journal-title":"Cancer Res"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/314633a0"},{"key":"e_1_2_1_14_1","first-page":"3974","article-title":"Centrosome defects and genetic instability in malignant tumors","volume":"58","author":"Pihan GA","year":"1998","journal-title":"Cancer Res"},{"key":"e_1_2_1_15_1","first-page":"1398","article-title":"Centrosome abnormalities and chromosome instability occur together in pre\u2010invasive carcinomas","volume":"63","author":"Pihan GA","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_1_16_1","article-title":"Geometric approach to segmentation and protein localization in cell culture assays","author":"Raman S","year":"2006","journal-title":"J Microsc"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1109\/TSMC.1978.4310039"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:JOMG.0000048774.27697.30"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/25.7.70454"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjemt.20372","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.20372","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T14:40:38Z","timestamp":1760625638000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.20372"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,8,29]]},"references-count":18,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2006,12]]}},"alternative-id":["10.1002\/jemt.20372"],"URL":"https:\/\/doi.org\/10.1002\/jemt.20372","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"type":"print","value":"1059-910X"},{"type":"electronic","value":"1097-0029"}],"subject":[],"published":{"date-parts":[[2006,8,29]]}}}