{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T05:35:52Z","timestamp":1788068152882,"version":"build-2784847793"},"reference-count":63,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2007,3,29]],"date-time":"2007-03-29T00:00:00Z","timestamp":1175126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This research has been designed to study the major events of nuclear remodeling that characterize sheep oocytes during the early stage of folliculogenesis (transition from preantral to antral stage). In particular, the modifications in large\u2010scale chromatin configuration, the global DNA methylation, and the process of telomere elongation have been investigated as crucial events of oocyte nuclear maturity. In addition, the spatio\u2010temporal distribution of the major enzymes involved in DNA methylation, the DNA methyltransferase 1 (Dnmt1), and in telomere elongation, telomerase catalytic subunit (TERT), have been described. To these aims, the nuclei of isolated oocytes were investigated using immunocytochemistry and Q\u2010FISH analyses. In absence of preliminary information, these nuclear determinants were compared with those of fully competent germ cells obtained from medium and preovulatory antral follicles. The nuclei of sheep oocytes acquired a condensed chromatin configuration, stable high levels of global DNA methylation, and a definitive telomere length already in the majority of late growing stage oocytes (110 \u03bcm) derived from early antral follicles. In addition, while the process of methylation resulted strictly related to oocyte diameter, the telomeric program appeared to be highly chromatin configuration\u2010dependent. The translocation of Dnmt1 and TERT from the nucleus to the cytoplasm in the oocytes derived from early antral follicles seems to confirm the definitive chromatin asset of these germ cells. In conclusion, changes in large\u2010scale chromatin structure, epigenesis, and telomere size in the sheep are established prior to oocyte acquires the ability to resume meiosis. Microsc. Res. Tech., 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jemt.20462","type":"journal-article","created":{"date-parts":[[2007,3,29]],"date-time":"2007-03-29T13:59:08Z","timestamp":1175176748000},"page":"733-744","source":"Crossref","is-referenced-by-count":33,"title":["Modifications in chromatin morphology and organization during sheep oogenesis"],"prefix":"10.1002","volume":"70","author":[{"given":"Valentina","family":"Russo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Martelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Berardinelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oriana","family":"Di 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