{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:35:32Z","timestamp":1760661332232,"version":"build-2065373602"},"reference-count":45,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2007,8,23]],"date-time":"2007-08-23T00:00:00Z","timestamp":1187827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nitric oxide (NO) has been implied in age\u2010related changes of the central nervous system (CNS) and the central auditory pathway. The present study was conducted to investigate whether the number of NO\u2010producing cells and their morphometric characteristics in the inferior colliculus (IC) and the auditory cortex (AC) are changed with the increasing age of the subjects. IC and AC sections of adult and senile Wistar rats were studied using the histochemical detection of NADPH\u2010diaphorase activity (NADPH\u2010d), a marker for neurons containing nitric oxide synthase (NOS). Our results showed a decreased area of the somas of NADPH\u2010d\u2010positive neurons in the dorsal cortex (DC) of the IC and a diffuse loss of NADPH\u2010d\u2010positive neurons in the senile IC and primary cortical auditory area (Te1). However, an increased number of NO\u2010producing cells have been shown by other authors in different parts of the ageing auditory pathway and CNS. It seems that age\u2010related changes in NADPH\u2010d\u2010positive cells may follow a region\u2010specific route. These changes may be related to hearing impairments with increasing age. Microsc. Res. 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