{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T20:41:41Z","timestamp":1773348101062,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2008,3,4]],"date-time":"2008-03-04T00:00:00Z","timestamp":1204588800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2008,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim was to survey articular calcified cartilage (ACC) and subchondral bone in the palmar and dorsal regions of the condyles of the third metacarpal bone (Mc3) of young horses with minimal or no signs of musculo\u2010skeletal abnormality. Back\u2010scattered electron scanning electron microscopy (BSE SEM) was conducted on polymethyl methacrylate\u2010embedded mediolateral slices and macerated wedges of the right distal Mc3 from seven each of trained and untrained 2\u2010year\u2010old Thoroughbred horses. Furrows or grooves visible to the naked eye in the mineralizing front (MF) of ACC are the commonest \u201clesion\u201d and are most common in the palmar portions of the medial and lateral condylar grooves. Cracks running predominantly in the parasagittal plane that infill with hypercalcified matrix are found in the same domain. Common to all these defects are deficiencies or absence of the ACC MF. Other anomalies include local excrescences or depressed areas of the MF. More important condylar lesions show displaced fragmented hypermineralized ACC with underlying excess resorption in the bone domain, leaving a thin ACC layer with cavernous space beneath it. The fragments may dislodge and displace to the joint space. Obvious although small lesions are present in horses that have undertaken little or no training. The nature and sites of the lesions indicate that they are possibly the earliest morphological evidence of changes that may lead to specific joint abnormalities. The lesions appear unlikely to be solely due to functional traumatic forces, and developmental influences are likely to be important in their initiation. Microsc. Res. Tech., 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jemt.20575","type":"journal-article","created":{"date-parts":[[2008,3,4]],"date-time":"2008-03-04T11:16:24Z","timestamp":1204629384000},"page":"477-488","source":"Crossref","is-referenced-by-count":26,"title":["High resolution microscopic survey of third metacarpal articular calcified cartilage and subchondral bone in the juvenile horse: Possible implications in chondro\u2010osseous disease"],"prefix":"10.1002","volume":"71","author":[{"given":"Alan","family":"Boyde","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elwyn C.","family":"Firth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,3,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1063-4584(03)00152-3"},{"key":"e_1_2_1_3_1","first-page":"251","volume-title":"Methods of calcified tissue preparation","author":"Boyde A","year":"1984"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.2003.00213.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.2003.00146.x"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0029(19960201)33:2<92::AID-JEMT2>3.0.CO;2-0"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0021-8782.2004.00354.x"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/00480169.2005.36489"},{"issue":"1","key":"e_1_2_1_9_1","first-page":"S296","article-title":"Increased matrix mineralisation of cartilage and bone in scurvy","volume":"12","author":"Boyde A","year":"1997","journal-title":"J Bone Miner Res"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1999.tb03805.x"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v009a05"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.2746\/042516400777584613"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1999.tb03794.x"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.2003.00193.x"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/00480169.2005.36488"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/00480169.2004.36438"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/00480169.2005.36487"},{"key":"e_1_2_1_18_1","first-page":"418","article-title":"Changes in diaphyseal and epiphyseal bone parameters in thoroughbred horses after withdrawal from training","volume":"6","author":"Firth EC","year":"2007","journal-title":"J Musculoskel Neuronal Interact"},{"key":"e_1_2_1_19_1","first-page":"151","article-title":"Microradiographic study of the calcified layer or articular cartilage","volume":"90","author":"Green WT","year":"1970","journal-title":"Arch Pathol"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-002-2010-9"},{"key":"e_1_2_1_21_1","first-page":"33","volume-title":"Diagnostic and surgical arthroscopy in the horse","author":"McIlwraith CW","year":"1990"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(97)00200-7"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2005.08.020"},{"key":"e_1_2_1_24_1","first-page":"251","article-title":"Subchondral bone failure in overload arthrosis: A scanning electron microscopic study in horses","volume":"6","author":"Norrdin RW","year":"2006","journal-title":"J Musculoskel Neuronal Interact"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(97)00253-6"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.4102\/jsava.v68i4.893"},{"key":"e_1_2_1_27_1","first-page":"87","volume-title":"Joint disease in the horse","author":"Pool R","year":"1996"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2003.64.1110"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1996.tb03777.x"},{"key":"e_1_2_1_30_1","first-page":"555","volume-title":"Equine exercise physiology 5","author":"Riggs CM","year":"1999"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1999.tb03806.x"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1999.tb03807.x"},{"key":"e_1_2_1_33_1","first-page":"191","article-title":"Micro\u2010cracks in the calcified layer of articular cartilage","volume":"117","author":"Sokoloff L","year":"1993","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_1_34_1","first-page":"183","article-title":"The influence of exercise on the composition of developing equine joints","volume":"39","author":"van de Lest CHA","year":"2002","journal-title":"Biorheology"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(03)00083-8"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjemt.20575","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.20575","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T18:32:20Z","timestamp":1760639540000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.20575"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,3,4]]},"references-count":34,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2008,6]]}},"alternative-id":["10.1002\/jemt.20575"],"URL":"https:\/\/doi.org\/10.1002\/jemt.20575","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"value":"1059-910X","type":"print"},{"value":"1097-0029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,3,4]]}}}