{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T11:00:00Z","timestamp":1761217200614,"version":"build-2065373602"},"reference-count":17,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2008,6,19]],"date-time":"2008-06-19T00:00:00Z","timestamp":1213833600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Peripheral nerve biopsies from 10 Lepromatous leprosy (LL) patients who were on multidrug treatment (MDT) were investigated by light and electron microscopy. Clofazimine (CLF) has been included as an essential component of MDT, which is the standard WHO regimen for treatment of leprosy. The patients receiving continuous MDT for a long period had viable bacilli in Schwann cells (SCs) of peripheral nerves whereas they had disappeared from the skin. Our ultrastructural observations clearly indicated the presence of CLF crystals in SCs. The crystals were in the form of osmiophilic rods of various shapes and sizes. On the other hand, the blood nerve barrier was clearly noticed in endoneurial blood vessels (EBV), and the barrier seems to play an important role for penetration of antileprosy drugs especially CLF. Microsc. Res. Tech., 2008 \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jemt.20604","type":"journal-article","created":{"date-parts":[[2008,7,24]],"date-time":"2008-07-24T14:35:07Z","timestamp":1216910107000},"page":"614-618","source":"Crossref","is-referenced-by-count":2,"title":["Does clofazimine (B663) reach <i>Mycobacterium leprae<\/i> persisting in Schwann cells and endothelial cells of endoneurial blood vessels in peripheral nerves?"],"prefix":"10.1002","volume":"71","author":[{"given":"V.","family":"Kumar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,6,19]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00690562"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00690394"},{"key":"e_1_2_1_4_1","first-page":"650","article-title":"Uptake by reticuloendothelial cells of the riminophenazine B663","volume":"423","author":"Conalty ML","year":"1962","journal-title":"Brit J Exp Pathol"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01742765"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00688442"},{"key":"e_1_2_1_7_1","first-page":"251","article-title":"Pathology of peripheral nerves lesion in lepromatous leprosy\u2014A light and electron microscopic study","volume":"39","author":"Job CK","year":"1971","journal-title":"Int J Leprosc"},{"key":"e_1_2_1_8_1","first-page":"29","article-title":"Ultrastructural demonstration of Clofazimine crystals in Schwann cells from lepromatous leprosy","volume":"68","author":"Kumar V","year":"1998","journal-title":"Microsc Anal"},{"key":"e_1_2_1_9_1","first-page":"328","article-title":"Ultrastructural study of Schwann cells and Endothelial cells in the pathogenesis of lepromatous neuropathy","volume":"71","author":"Kumar V","year":"2003","journal-title":"Int J Leprosc"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.5025\/hansen1977.58.179"},{"key":"e_1_2_1_11_1","first-page":"81","article-title":"An ultrastructural study of Schwann cells in peripheral nerves of leprosy patients","volume":"64","author":"Kumar V","year":"1992","journal-title":"Ind J Leprosc"},{"key":"e_1_2_1_12_1","first-page":"1","article-title":"Electron microscopic studies of the antileprosy drug B663 (Clofazimine lamprene)","volume":"42","author":"McDougall AC","year":"1974","journal-title":"Int J Leprosc"},{"key":"e_1_2_1_13_1","first-page":"355","article-title":"Classification of leprosy according to immunity. A five group system","volume":"34","author":"Ridley DS","year":"1966","journal-title":"Int J Leprosc"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)01335-6"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65414-4"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711140209"},{"key":"e_1_2_1_17_1","first-page":"472","article-title":"Tissue distribution and deposition of Clofazimine in mice following oral administration with or without isoniazid","volume":"57","author":"Venkatesan K","year":"2007","journal-title":"Arzneimittel\u2010forschung"},{"key":"e_1_2_1_18_1","first-page":"289","volume-title":"Proceedings of the Xth International Leprosy Congress","author":"Waters MFR","year":"1978"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjemt.20604","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.20604","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T14:01:06Z","timestamp":1760623266000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.20604"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,6,19]]},"references-count":17,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2008,8]]}},"alternative-id":["10.1002\/jemt.20604"],"URL":"https:\/\/doi.org\/10.1002\/jemt.20604","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"type":"print","value":"1059-910X"},{"type":"electronic","value":"1097-0029"}],"subject":[],"published":{"date-parts":[[2008,6,19]]}}}