{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:40:26Z","timestamp":1789429226626,"version":"build-2803163510"},"reference-count":18,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2008,9,10]],"date-time":"2008-09-10T00:00:00Z","timestamp":1221004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>By providing two examples, the option for embedding 3D models in electronic versions of life science publications is presented. These examples, presumably representing the first such models published, are developmental stages of an evertebrate (<jats:italic>Patella caerulea,<\/jats:italic> Mollusca) and a vertebrate species (<jats:italic>Psetta maxima<\/jats:italic>, Teleostei) obtained from histological section series reconstruction processed with the software package Amira. These surface rendering models are particularly suitable for a PDF file because they can easily be transformed to a file format required and components may be conveniently combined and hierarchically arranged. All methodological steps starting from specimen preparation until embedding of resulting models in PDF files with emphasis on conversion of Amira data to the appropriate 3D file format are explained. Usability of 3D models in PDF documents is exemplified and advantages over 2D illustrations are discussed, including better explanation capabilities for spatial arrangements, higher information contents, and limiting options for disguising results by authors. Possibilities for additional applications reaching far beyond the examples presented are suggested. Problems such as long\u2010term compatibility of file format and hardware plus software, editing and embedding of files, file size and differences in information contents between printed and electronic version will likely be overcome by technical development and increasing tendency toward electronic at the cost of printed publications. Since 3D visualization plays an increasing role in manifold disciplines of science and appropriate tools for the popular PDF format are readily available, we propose routine application of this way of illustration in electronic life science papers. Microsc. Res. Tech., 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jemt.20618","type":"journal-article","created":{"date-parts":[[2008,9,10]],"date-time":"2008-09-10T14:44:24Z","timestamp":1221057864000},"page":"778-786","source":"Crossref","is-referenced-by-count":129,"title":["Embedding 3D models of biological specimens in PDF publications"],"prefix":"10.1002","volume":"71","author":[{"given":"Bernhard","family":"Ruthensteiner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"He\u00df","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,9,10]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8649.1997.tb01650.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.newast.2008.03.008"},{"key":"e_1_2_1_4_1","unstructured":"GrundmannC He\u00dfM.2007. Pseudo 4D reconstruction of the flatfish head during metamorphosis in the turbotPsetta maxima.100. Annual meeting of the German Zoological Society Cologne Germany September 21\u201324 2007 abstract book: 184. Available at:http:\/\/www.uni\u2010koeln.de\/math\u2010nat\u2010fak\/zoologie\/dzg07\/Abstractbook.pdf. Accessed January 8 2008."},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.zool.2005.09.008"},{"key":"e_1_2_1_6_1","unstructured":"KochP He\u00dfM.2006. Pseudo\u20104D\u2010visualization of a flatfish head during metamorphosis. 99. Annual meeting of the German Zoological Society M\u00fcnster Germany September 16\u201320 2006 abstract book: 137. Available at:http:\/\/www.dzg\u2010ev.de\/de\/jahrestagung\/2006_muenster_99\/abstractband_muenster2006.pdf. Accessed January 8 2008."},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903030408"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-142X.2007.00157.x"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.5194\/bg-5-25-2008"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.10398"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/447138a"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1096-3642.1997.tb00137.x"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200406019"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/mollus\/eyl019"},{"key":"e_1_2_1_15_1","first-page":"1","article-title":"Soft part 3D visualization by serial sectioning and computer reconstruction","author":"Ruthensteiner B","year":"2008","journal-title":"Zoosymposia"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004350100040"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1385-299X(03)00055-2"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004270050247"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1111\/j.1744-7410.2000.tb00006.x","article-title":"Torsion in Patella caerulea (Mollusca, Patellogastropoda): Ontogenetic process, timing, and mechanisms","volume":"119","author":"Wanninger A","year":"2000","journal-title":"Invertebr Biol"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjemt.20618","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.20618","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T16:26:15Z","timestamp":1760631975000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.20618"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,9,10]]},"references-count":18,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2008,11]]}},"alternative-id":["10.1002\/jemt.20618"],"URL":"https:\/\/doi.org\/10.1002\/jemt.20618","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"value":"1059-910X","type":"print"},{"value":"1097-0029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,9,10]]}}}