{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T19:43:25Z","timestamp":1760643805110,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,11,25]],"date-time":"2009-11-25T00:00:00Z","timestamp":1259107200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Macrophages from smokers demonstrate an increased auto\u2010fluorescence. Similarly, auto\u2010fluorescence follows in vitro exposure of macrophages to cigarette smoke condensate (i.e., the particulate fraction of cigarette smoke). The composition of particles in cigarette smoke can be comparable to air pollution particles. We tested the postulate that macrophages exposed to air pollution particles could demonstrate auto\u2010fluorescence. Healthy nonsmoking and healthy smoking volunteers (both 18\u201340 years of age) underwent fiberoptic bronchoscopy with bronchoalveolar lavage and alveolar macrophages isolated. Macrophages were incubated at 37\u00b0C in 5% CO<jats:sub>2<\/jats:sub> with either PBS or 100 \u03bcg\/mL particle for both 1 and 24 h. Particles included a residual oil fly ash, Mt. St. Helens volcanic ash, and ambient air particles collected from St. Louis, Missouri and Salt Lake City, Utah. At the end of incubation, 50 \u03bcL of the cell suspension was cytocentrifuged and examined at modes for viewing fluorescein isothiocyanate (FITC) and rhodamine fluorescence. Both emission source air pollution particles demonstrated FITC and rhodamine auto\u2010fluorescence at 1 and 24 h, but the signal following incubation of the macrophages with oil fly ash appeared greater. Similarly, the ambient particles were associated with auto\u2010fluorescence by the alveolar macrophages and this appeared to be dose\u2010dependent. We conclude that exposure of macrophages to air pollution particles can be associated with auto\u2010fluorescence in the FITC and rhodamine modes. Microsc. Res. Tech., 2010. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jemt.20804","type":"journal-article","created":{"date-parts":[[2009,11,25]],"date-time":"2009-11-25T19:42:57Z","timestamp":1259178177000},"page":"579-582","source":"Crossref","is-referenced-by-count":3,"title":["MRT letter: Auto\u2010fluorescence by human alveolar macrophages after in vitro exposure to air pollution particles"],"prefix":"10.1002","volume":"73","author":[{"given":"Andrew J.","family":"Ghio","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rahul G.","family":"Sangani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luisa E.","family":"Brighton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John 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