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At present, analysis methods are limited in their ability to allow for this simultaneous analysis of bone and bone vasculature in three dimensions, without using the most recent dual\u2010energy computed tomography (CT) techniques. In this study, we present a new barium sulfate (BaSO<jats:sub>4<\/jats:sub>) radiopaque vascular perfusion compound for performing postmortem microangiography with single\u2010beam microcomputed tomography (microCT), which allows for such simultaneous analysis. This compound differs from currently available contrast mediums due to (1) the high weight\u2010to\u2010volume ratio of BaSO<jats:sub>4<\/jats:sub> achieved, (2) small BaSO<jats:sub>4<\/jats:sub> aggregate size (&lt;5 \u03bcm), (3) minimal additives, and (4) its miscibility with blood and saline. Most notably, it achieves a radiodensity of 2.4\u00d7 that of cortical bone, with high perfusion of both the arterial and venous systems and the intervening capillary bed, resulting in an in vivo radiodensity that ranges from that of bone to titanium. Our results, verified using a rat femoral gap\u2010healing model, show that the compound is uniquely suited to high\u2010contrast imaging of the vasculature in the presence of undecalcified bone, with a versatility to be used in other tissues. Microsc. Res. Tech., 2010. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jemt.20807","type":"journal-article","created":{"date-parts":[[2009,11,25]],"date-time":"2009-11-25T19:53:32Z","timestamp":1259178812000},"page":"665-672","source":"Crossref","is-referenced-by-count":12,"title":["A new bone vascular perfusion compound for the simultaneous analysis of bone and vasculature"],"prefix":"10.1002","volume":"73","author":[{"given":"Krista L.","family":"Sider","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janice","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John 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