{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T20:18:29Z","timestamp":1771964309395,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2011,4,20]],"date-time":"2011-04-20T00:00:00Z","timestamp":1303257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Aedes aegypti<\/jats:italic> is the principal vector of dengue world wide and a major vector of urban yellow fever. Despite its epidemiological importance, not much is known regarding cellular and structural changes in the fat body in this mosquito. Here, we applied light and transmission electron microscopies to investigate structural changes in the fat body of three groups of <jats:italic>A. aegypti<\/jats:italic> females: newly emerged, 18\u2010day\u2010old sugar\u2010fed, and 18\u2010day\u2010old blood\u2010fed. The fat body consists of a layer of cells attached to the abdomen integument, formed by trophocytes and oenocytes. Trophocytes are strongly positive for carbohydrates, while oenocytes are strongly positive for proteins and lipids. Ultrastructural analyses of trophocytes from newly emerged and 18\u2010day\u2010old blood\u2010fed indicate that these cells are rich in glycogen and free ribosomes. Many lipid droplets and protein granules, which are broken down after the blood meal, are also detected. In 18\u2010day\u2010old sugar\u2010fed, trophocytes display a disorganized cytoplasm filled with lipid droplets, and reduced numbers of free ribosomes, glycogen, rough endoplasmic reticulum (RER) and mitochondria. Following a blood meal, the RER and mitochondria display enlarged sizes, suggestive of increased activity. With regard to oenocytes, these cells display an electron\u2010dense cytoplasm and plasma membrane infoldings facing the hemolymph. As the <jats:italic>A. aegypti<\/jats:italic> female ages, trophocyte and oenocyte cell nuclei become larger but decrease in diameter after blood feeding. Our findings suggest that the trophocytes and oenocytes remodeling is likely involved in functional changes of fat body that take place during aging and following a blood meal in <jats:italic>A. aegypti<\/jats:italic> females. Microsc. Res. Tech. 2011. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jemt.20990","type":"journal-article","created":{"date-parts":[[2011,4,20]],"date-time":"2011-04-20T17:12:00Z","timestamp":1303319520000},"page":"1032-1039","source":"Crossref","is-referenced-by-count":28,"title":["Histochemical and ultrastructural studies of the mosquito <i>Aedes aegypti<\/i> fat body: Effects of aging and diet type"],"prefix":"10.1002","volume":"74","author":[{"given":"Gustavo Ferreira","family":"Martins","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Eduardo","family":"Serr\u00e3O","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Marcelo","family":"Ramalho\u2010Ortig\u00e3O","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Filemon Paolucci","family":"Pimenta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,4,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00224938"},{"key":"e_1_2_6_3_1","first-page":"241","volume-title":"Manual de t\u00e9cnicas para histologia normal e patol\u00f3gica","author":"Behmer OA","year":"1976"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402390110"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0040-8166(88)90047-X"},{"key":"e_1_2_6_6_1","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1017\/CBO9780511818202","volume-title":"The insects. 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