{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T03:16:57Z","timestamp":1771298217152,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,7,14]],"date-time":"2011-07-14T00:00:00Z","timestamp":1310601600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study analyzed the newly formed bone tissue after application of recombinant human BMP\u20102 (rhBMP\u20102) and P\u20101 (extracted from <jats:italic>Hevea brasiliensis<\/jats:italic>) proteins, 2 weeks after the creation of a critical bone defect in male Wistar rats treated or not with a low\u2010intensity laser (GaAlAs 780 nm, 60 mW of power, and energy density dose of 30 J\/cm<jats:sup>2<\/jats:sup>). The animals were divided into two major groups: (1) bone defect plus low\u2010intensity laser treatment and (2) bone defect without laser irradiation. The following subgroups were also analyzed: (a) 5 \u03bcg of pure rhBMP\u20102; (b) 5 \u03bcg of pure P\u20101 fraction; (c) 5 \u03bcg of rhBMP\u20102\/monoolein gel; (d) 5 \u03bcg of P\u20101 fraction\/monoolein gel; (e) pure monoolein gel. Comparisons of the groups receiving laser treatment with those that did not receive laser irradiation show differences in the areas of new bone tissue. The group treated with 5 \u03bcg of rhBMP\u20102 and laser irradiation was not significantly different (<jats:italic>P<\/jats:italic> &gt;0.05) than the nonirradiated group that received the same treatment. The irradiated, rhBMP\u20102\/monoolein gel treatment group showed a lower area of bone formation than the nonirradiated, rhBMP\u20102\/gel monoolein treatment group (<jats:italic>P<\/jats:italic> &lt; 0.001). The area of new bone tissue in the other nonirradiated and irradiated groups was not significantly different (<jats:italic>P<\/jats:italic> &gt; 0.05). Furthermore, the group that received the 5 \u03bcg of rhBMP\u20102 application showed the greatest bone formation. We conclude that the laser treatment did not interfere with the area of new bone tissue growth and that the greatest stimulus for bone formation involved application of the rhBMP\u20102 protein. Microsc. Res. Tech. 2011. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jemt.21033","type":"journal-article","created":{"date-parts":[[2011,7,15]],"date-time":"2011-07-15T00:21:37Z","timestamp":1310689297000},"page":"117-125","source":"Crossref","is-referenced-by-count":9,"title":["Influence of low\u2010level laser associated with osteogenic proteins recombinant human BMP\u20102 and <i>Hevea brasiliensis<\/i> on bone repair in Wistar rats"],"prefix":"10.1002","volume":"75","author":[{"given":"Mamie Mizusaki","family":"Iyomasa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Paulo","family":"Mardegan Issa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirella Lemos","family":"de Queir\u00f3z Tavares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yamba Carla","family":"Lara 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