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We report a study on the model of the intestine of <jats:italic>Isospora suis<\/jats:italic>\u2010infected piglets, in which we addressed (1) whether the quantity of detectable T cells in the intestinal mucosa is the same in formalin\u2010, HOPE\u00ae\u2010, and cryo\u2010conserved material or whether the amounts of T cells at least correlate with one another; and (2) whether single jejunal segments differ in regard to the quantity of mucosal T cells and variability of lymphocyte infiltration. Quantification of T cells in histological sections of different parts of the jejunum of 15\u201022 day old piglets infected with <jats:italic>I. suis<\/jats:italic> was performed using an anti\u2010CD3\u2010antibody and stereological point counting. Area fractions of T\u2010cell profiles per intestinal mucosa profile were higher in cryo\u2010conserved samples than in HOPE\u00ae\u2010 and formalin\u2010conserved material but no correlation between different fixations could be found. The proximal part of the jejunum contained fewer T cells compared with mid\u2010 and end\u2010jejunum. Coefficients of variation did not differ between the intestinal segments. For quantification of T cells in the gut mucosa of piglets infected with <jats:italic>I. suis<\/jats:italic>, the cryo\u2010conserved mid jejunum seems most suitable in cases when unbiased sampling of the complete intestine is not feasible. It is generally not possible to compare quantitative results of immunostaining in samples conserved by different methods. Microsc. Res. Tech., 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jemt.21071","type":"journal-article","created":{"date-parts":[[2011,9,7]],"date-time":"2011-09-07T04:40:32Z","timestamp":1315370432000},"page":"408-415","source":"Crossref","is-referenced-by-count":9,"title":["Immunohistochemical detection and quantification of T cells in the small intestine of <i>Isospora suis<\/i>\u2010infected piglets\u2014influence of fixation technique and intestinal segment"],"prefix":"10.1002","volume":"75","author":[{"given":"Simone","family":"Gabner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zbyn\u011bk","family":"Tonar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Tichy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Armin","family":"Saalm\u00fcller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanna Lucia","family":"Worliczek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anja","family":"Joachim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kirsti","family":"Witter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,9,7]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10014-010-0004-2"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-008-0458-z"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.33579"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2009.02.001"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2010.234"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2818.2000.00750.x"},{"key":"e_1_2_5_8_1","unstructured":"GaiswinklerC.2010. 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