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Such structures are known to occur in basophilic cells of gastropod digestive gland, where they are supposed to be involved in the regulation of some minerals and in detoxification. In this study, energy\u2010dispersive X\u2010ray analysis (EDX) and energy filtered transmission electron microscopy (EFTEM) were used to determine the elemental content of spherocrystals in two Strombidae, <jats:italic>Strombus gigas<\/jats:italic> and <jats:italic>Strombus pugilis<\/jats:italic>. In freshly collected individuals of both species, the following elements were detected: Ca, Fe, Mg, P, and Zn. Aluminum and Mn were also detected in <jats:italic>S. gigas.<\/jats:italic> Their presence in spherocrystals indicates that, in Strombidae, spherocrystals are involved in the regulation of minerals and essential trace metals. In order to answer the question \u201care spherocrystals involved in nonessential trace metals scavenging?,\u201d artificial cadmium and lead exposure by both waterborne and dietary pathways was applied to <jats:italic>S. pugilis<\/jats:italic>. No evidence of cadmium (Cd(NO<jats:sub>3<\/jats:sub>)<jats:sub>2<\/jats:sub>) or lead (Pb(NO<jats:sub>3<\/jats:sub>)<jats:sub>2<\/jats:sub>) provided by food was found in spherocrystals. Cadmium provided in water (Cd(NO<jats:sub>3<\/jats:sub>)<jats:sub>2<\/jats:sub> and CdCl<jats:sub>2<\/jats:sub>) causes structural modifications of the digestive gland; however, this element was not trapped in spherocrystals. These results suggest that spherocrystals are not involved in detoxification of such nonessential trace metals. Microsc. Res. Tech., 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jemt.21073","type":"journal-article","created":{"date-parts":[[2011,9,14]],"date-time":"2011-09-14T09:24:17Z","timestamp":1315992257000},"page":"425-432","source":"Crossref","is-referenced-by-count":10,"title":["Insight of EDX analysis and EFTEM: Are spherocrystals located in strombidae digestive gland implied in detoxification of trace metals?"],"prefix":"10.1002","volume":"75","author":[{"given":"Jean\u2010Marie","family":"Volland","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Pierre","family":"Lechaire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghislaine","family":"Frebourg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalila","family":"Aldana 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