{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T23:11:25Z","timestamp":1781478685290,"version":"3.54.1"},"reference-count":27,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,2,24]],"date-time":"2012-02-24T00:00:00Z","timestamp":1330041600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Chrysomya megacephala<\/jats:italic> is a forensic important fly, and its immature forms also cause myiasis. The adults are the first insects to reach a carcass and can oviposit just a few hours after arrival. Therefore, the knowledge of immature stages of flies is essential for correct identification of the species found on corpses. Scanning electron microscopy (SEM) gives detailed information on the morphological characters that can help identify the immature forms of flies. The aim of this study is to identify larvae and puparia of <jats:italic>C. megacephala<\/jats:italic> using SEM. The larval instar body of <jats:italic>C. megacephala<\/jats:italic> is similar at all instars. The integument is smooth with small spines located at the limit of all segments. The cephalic region has a group of robust spines with one or two tips. The puparia are very similar to third instar larvae, except for the cephalic structures that are retracted. The integument shows the wrinkles from the third instar larvae and posterior spiracle disc with three spiracular openings localized on the top of an elevation. In conclusion, SEM provides some characteristics to distinguish among <jats:italic>Chrysomya<\/jats:italic> species that could help entomologists to identify immature found on corpses. Microsc. Res. Tech. 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jemt.22017","type":"journal-article","created":{"date-parts":[[2012,2,24]],"date-time":"2012-02-24T05:40:44Z","timestamp":1330062044000},"page":"935-939","source":"Crossref","is-referenced-by-count":21,"title":["Ultrastructure of larvae and puparia of the blowfly <i>Chrysomya megacephala<\/i> (Diptera: Calliphoridae)"],"prefix":"10.1002","volume":"75","author":[{"given":"Paloma Martins","family":"Mendon\u00e7a","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacenir Reis","family":"Dos Santos\u2010Mallet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margareth Maria","family":"De Carvalho Queiroz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,2,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762001000600007"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1520\/JFS14567J"},{"key":"e_1_2_6_4_1","first-page":"440","volume-title":"Forensic entomology: The utility of arthropods in legal investigations","author":"Byrd JH","year":"2001"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2915.1989.tb00229.x"},{"key":"e_1_2_6_6_1","first-page":"487","article-title":"First record of human myiasis caused by association of the species Chrysomya megacephala (Diptera: Calliphoridae), Sarcophaga (Liopygia) ruficornis (Diptera: Sarcophagidae) and Musca domestica (Diptera: Muscidae)","volume":"47","author":"Ferraz ACP","year":"2010","journal-title":"Entomol Soc Am"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s00414-002-0323-x","article-title":"The blowfly Chrysomya albiceps (Wiedemann) (Diptera: Calliphoridae) as a new Forensic Indicator in Central Europe","volume":"117","author":"Grassberg M","year":"2003","journal-title":"Int J Legal Med"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/32.1.21"},{"key":"e_1_2_6_9_1","volume-title":"Myiasis in man and animals in the Neotropical region","author":"Guimar\u00e3es JH","year":"1999"},{"key":"e_1_2_6_10_1","first-page":"245","article-title":"Dispersal and distribution of three newly introduced species of Chrysomya R.D. 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