{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:28:48Z","timestamp":1760660928212,"version":"build-2065373602"},"reference-count":13,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,4,11]],"date-time":"2013-04-11T00:00:00Z","timestamp":1365638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The aim of this study was to investigate the effect of Sibutramine on platelet ultrastructure and discuss the morphological observations in relation to known physiological effects of the compound. Six\u2010week\u2010old, female Spraque\u2010Dawley rats were used in this study. The animals were placed on a high energy diet after which sibutramine administration followed. Blood was drawn on the day of termination and platelet rich plasma was obtained to prepare plasma smears for analysis. Scanning electron microscopy was used to investigate the ultrastructure of the platelets. Platelets of the Sibutramine\u2010treated animals showed smooth surface with limited pseudopodia formation when compared with that of the control animals. Higher magnification of the platelet surface showed membrane tears and swelling, typically seen in necrotic cells. It can therefore be concluded from these results that Sibutramine alters the membrane morphology of platelets to that typical of necrotic cells. <jats:italic>Microsc. Res. Tech. 76:653\u2013657, 2013<\/jats:italic>. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jemt.22214","type":"journal-article","created":{"date-parts":[[2013,4,11]],"date-time":"2013-04-11T02:32:54Z","timestamp":1365647574000},"page":"653-657","source":"Crossref","is-referenced-by-count":0,"title":["The effect of sibutramine on platelet morphology of Spraque\u2010Dawley rats fed a high energy diet"],"prefix":"10.1002","volume":"76","author":[{"given":"Hester Magdalena","family":"Oberholzer","sequence":"first","affiliation":[{"name":"Department of Anatomy Faculty of Health Sciences University of Pretoria  South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ciska","family":"Van Der Schoor","sequence":"additional","affiliation":[{"name":"Department of Anatomy Faculty of Health Sciences University of Pretoria  South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Etheresia","family":"Pretorius","sequence":"additional","affiliation":[{"name":"Department of Physiology Faculty of Health Sciences University of Pretoria  South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,4,11]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/oby.2003.186"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1587"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2007.04542.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874609810801010051"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2011.11.030"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-789X.2007.00425.x"},{"key":"e_1_2_5_8_1","first-page":"782","article-title":"Function of glycoprotein VI and integrin alpha2beta1 in the procoagulant response of single, collagen\u2010adherent platelets","volume":"5","author":"Heemskerk JW","year":"1999","journal-title":"Thromb Haemostasis"},{"key":"e_1_2_5_9_1","doi-asserted-by":"crossref","first-page":"2615","DOI":"10.1182\/blood.V90.7.2615","article-title":"Collagen but not fibrinogen surfaces induce bleb formation, exposure of phosphatidylserine, and procoagulant activity of adherent platelets: Evidence for regulation by protein tyrosine kinase\u2010dependent Ca2+ responses","volume":"7","author":"Heemskerk JW","year":"1997","journal-title":"Blood"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/oby.2003.152"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(89)90048-8"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1124\/mi.10.4.6"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11239-010-0538-5"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07-9574LSF"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjemt.22214","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.22214","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T15:44:19Z","timestamp":1760629459000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.22214"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4,11]]},"references-count":13,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["10.1002\/jemt.22214"],"URL":"https:\/\/doi.org\/10.1002\/jemt.22214","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"type":"print","value":"1059-910X"},{"type":"electronic","value":"1097-0029"}],"subject":[],"published":{"date-parts":[[2013,4,11]]}}}