{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T16:12:32Z","timestamp":1760631152706,"version":"build-2065373602"},"reference-count":24,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,8,20]],"date-time":"2013-08-20T00:00:00Z","timestamp":1376956800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The morphology of cells and expression status of specific surface markers [cluster of differentiation (CD)], such as CD5, CD19, CD20, CD38, and CD45, have long been considered as the essential indicators for the diagnosis and prognosis of B\u2010cell chronic lymphocytic leukemia (B\u2010CLL). Clinically, it is difficult to simultaneously obtain cell morphology and distribution of surface markers with flow cytometry, especially for some surrogate markers such as CD38. Here, as an alternative and complementary prognostic method, fluorescence microscopy and image processing method are introduced to directly visualize the cells from patients and to quantitatively determine the expression status of surface markers. In this study, the morphological parameters of B\u2010CLL cells were measured to establish the correlation between the cellular morphology and the surface marker expression. It was clear that the CD38+ and CD38\u2212 B\u2010CLL cells from the same CD38+ patients had hardly any size differences; however, an increase in perimeter was observed for CD38\u2212 patients. Moreover, the expression level of the receptors on the cell was independent of the cell size. There was no evidence showing that the expression intensities of CD19 and CD38 were related to each other for the CD38+ B\u2010CLL cells. On the same cells, CD5 was more selectively expressed on the cell membrane; however, the expression patterns suggested that the cell membrane of CD38\u2212 B\u2010CLL cells contained the least expression level of CD19. <jats:italic>Microsc. Res. Tech. 76:1147\u20131153, 2013<\/jats:italic>. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jemt.22278","type":"journal-article","created":{"date-parts":[[2013,8,20]],"date-time":"2013-08-20T22:40:10Z","timestamp":1377038410000},"page":"1147-1153","source":"Crossref","is-referenced-by-count":1,"title":["Morphology and expression status investigations of specific surface markers on B\u2010cell chronic lymphocytic leukemia cells"],"prefix":"10.1002","volume":"76","author":[{"given":"Suli","family":"Niu","sequence":"first","affiliation":[{"name":"National Research Council Canada Ottawa Ontario Canada K1A 0R6"},{"name":"Department of Chemical and Biological Engineering University of Ottawa Ottawa Ontario Canada K1N 6N5"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan","family":"Chan","sequence":"additional","affiliation":[{"name":"National Research Council Canada Ottawa Ontario Canada K1A 0R6"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Berini","sequence":"additional","affiliation":[{"name":"School of Information Technology and Engineering University of Ottawa Ottawa Ontario Canada K1N 6N5"},{"name":"Department of Physics University of Ottawa Ottawa Ontario Canada K1N 6N5"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Mount Sinai Hospital Toronto Ontario Canada M5X 1G5"},{"name":"Faculty of Medicine University of Toronto Toronto Ontario Canada M5X 1G5"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan","family":"Zou","sequence":"additional","affiliation":[{"name":"National Research Council Canada Ottawa Ontario Canada K1A 0R6"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,8,20]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/hon.877"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830400404"},{"key":"e_1_2_5_4_1","first-page":"413","article-title":"Quantitative analysis of CD79b, CD5 and CD19 in mature B\u2010cell lymphoproliferative disorders","volume":"84","author":"Cabezudo E","year":"1999","journal-title":"Haematologica"},{"key":"e_1_2_5_5_1","doi-asserted-by":"crossref","first-page":"2615","DOI":"10.1182\/blood.V87.7.2615.bloodjournal8772615","article-title":"B\u2010chronic lymphocytic leukemia: A malignancy of anti\u2010self B cells","volume":"87","author":"Caligaris\u2010Cappio F","year":"1996","journal-title":"Blood"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beha.2007.02.005"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/3-540-29933-5_7"},{"key":"e_1_2_5_8_1","unstructured":"ChiorazziN DamleRN WasilT.2010.CD38 as a prognostic indicator in B cell chronic lymphocytic leukemia. 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