{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T16:12:10Z","timestamp":1760631130213,"version":"build-2065373602"},"reference-count":47,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,8,23]],"date-time":"2013-08-23T00:00:00Z","timestamp":1377216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Ticks of <jats:italic>Rhipicephalus sanguineus<\/jats:italic> species have great medical and veterinary importance for being a vector of various diseases. In an attempt to minimize their action on the host, people have resorted to chemical control by using various acaricides, such as selamectin. Although previous studies have demonstrated its toxic action in domestic animals, no studies focused on the detection of cell death when exposed to selamectin. For this reason, the technique for detecting autophagic cell death was used in order to demonstrate the responses of rabbits' skin tissues pre\u2010infested with <jats:italic>R. sanguineus<\/jats:italic> and exposed to different concentrations of selamectin. The obtained results when exposed to 100 and 80% concentrations of selamectin showed a strong mark of acid phosphatase on the cells of the connective tissue of the dermis and hair follicles, whereas the ones exposed to the 50% concentration had a weak mark on the cells of the connective tissue of the dermis and moderate staining in hair follicles. It became clear that, when used at high concentrations (100 and 80%), selamectin is capable to induce a large scale occurrence of the autophagic cell death process. On the other hand, the concentration of 50% causes minor morphophysiological changes in the skin of rabbit hosts when evaluated the cell death process. Therefore, the data confirms that selamectin is a powerful dose\u2010dependent toxic agent causes increased activity of the enzyme acid phosphatase. <jats:italic>Microsc. Res. Tech. 76:1171\u20131176, 2013<\/jats:italic>. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jemt.22281","type":"journal-article","created":{"date-parts":[[2013,8,23]],"date-time":"2013-08-23T18:24:39Z","timestamp":1377282279000},"page":"1171-1176","source":"Crossref","is-referenced-by-count":2,"title":["The occurrence of autophagic cell death in the tegument of rabbits pre\u2010infested with <i>Rhipicephalus sanguineus<\/i> and exposed to selamectin (active principle of acaricide pfizer revolution<sup>\u00ae<\/sup>)"],"prefix":"10.1002","volume":"76","author":[{"given":"Vlamir","family":"Bozzatto","sequence":"first","affiliation":[{"name":"Universidade Estadual Paulista (UNESP) Avenida 24A, 1515, Bela Vista CEP 13506\u2010900, CP 199 Rio Claro, SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patr\u00cdCia Rosa De","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista (UNESP) Avenida 24A, 1515, Bela Vista CEP 13506\u2010900, CP 199 Rio Claro, SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karim Christina Scopinho","family":"Furquim","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista (UNESP) Avenida 24A, 1515, Bela Vista CEP 13506\u2010900, CP 199 Rio Claro, SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Izabel","family":"Camargo\u2010Mathias","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista (UNESP) Avenida 24A, 1515, Bela Vista CEP 13506\u2010900, CP 199 Rio Claro, SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,8,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/01647954.2011.638320"},{"key":"e_1_2_7_3_1","first-page":"160","article-title":"Bloodsucking ticks (Ixodoidea)\u2010Vectors of diseases of man and animals","volume":"8","author":"Balashov YS","year":"1972","journal-title":"Miscell Publ Entom Soc Am"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.2460\/javma.1999.215.12.1813","article-title":"Respiratory failure attributable to moxidectin intoxication in a dog","volume":"215","author":"Beal MW","year":"1999","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_2_7_5_1","first-page":"61","article-title":"Rhipicephalus sanguineus tick in Brazil: feeding and reproductive aspects under laboratorial conditions","volume":"4","author":"Bechara GH","year":"1995","journal-title":"Brazil J Vet Parasitol"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-013-3422-3"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2009.12.013"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199908123410706"},{"key":"e_1_2_7_9_1","article-title":"Biology and ecology of the brown dog tick, Rhipicephalus sanguineus","volume":"3","author":"Dantas\u2010Torres F","year":"2010","journal-title":"Para Vect"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/352760880X"},{"key":"e_1_2_7_11_1","first-page":"104","volume-title":"\u00c1caros de import\u00e2ncia m\u00e9dico\u2010veterin\u00e1ria","author":"Flechtmann CHW","year":"1973"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2008.04.012"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0070-2153(06)78006-1"},{"key":"e_1_2_7_14_1","first-page":"24","article-title":"Bi\u00f3psia muscular com estudo histoqu\u00edmico: Experi\u00eancia inicial da Faculdade de Medicina de S\u00e3o Jos\u00e9 do Rio Preto","volume":"15","author":"Gra\u00e7a CR","year":"2008","journal-title":"Arq Ci\u00eanc Sa\u00fade"},{"key":"e_1_2_7_15_1","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.2460\/javma.1995.206.08.1147","article-title":"Invermectin toxicosis in two Australian shepherds","volume":"206","author":"Hadrick MK","year":"1995","journal-title":"J. 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