{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T14:44:25Z","timestamp":1770993865898,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2013,10,7]],"date-time":"2013-10-07T00:00:00Z","timestamp":1381104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The palatine mucosa and filiform papillae of the dorsal tongue mucosae of rodents were examined using transmission electron microscopy (TEM) and high resolution scanning electron microscopy (HRSEM). In the HRSEM method, the samples were fixed in 2% osmium tetroxide, dehydrated in alcohol, critical point\u2010dried, and coated with gold\u2010palladium. In addition, the HRSEM technique was used for morphometric analysis (length, width, and length\/width ratio of cocci and bacilli). For the TEM method, the tissues were fixed in modified Karnovsky solution (2.5% glutaraldehyde, 2% formalin in 0.1<jats:italic>M<\/jats:italic> sodium phosphate buffer, pH 7.4) and embedded in Spurr resin. The results demonstrated that there are thick polygonal keratinized epithelial cells where groups of bacteria are revealed in three\u2010dimensional images on the surface of filiform papillae in these animals. The bacterial membranes are randomly attached to the microplicae surface of epithelial cells. Morphometrics showed higher values of length and width of cocci in newborn (0 day) as compared to newborn (7 days) and adults animals, the bacilli showed no differences in these measurements. At high magnification, the TEM images revealed the presence of glycocalyx microfilaments that constitute a fine adhesion area between bacterial membranes and the membranes of epithelial microplicae cells. In conclusion, the present data revealed the fine fibrillar structures of bacteria that facilitate adhesion to the epithelial cell membranes of the oral cavity and morphometric changes in newborn (0 day) rats as compared with other periods. <jats:italic>Microsc. Res. Tech. 76:1226\u20131233, 2013<\/jats:italic>. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jemt.22289","type":"journal-article","created":{"date-parts":[[2013,10,11]],"date-time":"2013-10-11T22:37:47Z","timestamp":1381531067000},"page":"1226-1233","source":"Crossref","is-referenced-by-count":13,"title":["Fine Structure of Bacterial Adhesion to the Epithelial Cell Membranes of the Filiform Papillae of Tongue and Palatine Mucosa of Rodents: A Morphometric, TEM, and HRSEM Study"],"prefix":"10.1002","volume":"76","author":[{"given":"Ii\u2010Sei","family":"Watanabe","sequence":"first","affiliation":[{"name":"Department of Anatomy Institute of Biomedical Sciences, University of S\u00e3o Paulo S\u00e3o Paulo Brazil"},{"name":"Faculty of Veterinary Medicine and Animal Science, Department of Surgery University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koichi","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Department of Anatomy School of Medicine, Fukuoka University Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego Pulzatto","family":"Cury","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine and Animal Science, Department of Surgery University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando Jos\u00e9","family":"Dias","sequence":"additional","affiliation":[{"name":"Department of Anatomy Institute of Biomedical Sciences, University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcia Consentino Kronka","family":"Sosthenes","sequence":"additional","affiliation":[{"name":"Laboratory of Investigations in Neurodegeneration and Infection, University Hospital \u201cJo\u00e3o de Barros Barreto,\u201d Federal University of Par\u00e1 Bel\u00e9m Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Paulo Mardegan","family":"Issa","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, Department of Morphology Stomatology and Physiology, University of S\u00e3o Paulo Ribeir\u00e3o Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mamie Mizusaki","family":"Iyomasa","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, Department of Morphology Stomatology and Physiology, University of S\u00e3o Paulo Ribeir\u00e3o Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,10,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(71)90212-3"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345730520053701"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345750540041401"},{"key":"e_1_2_6_5_1","volume-title":"Oral microbiology and infectious disease","author":"Burnett GW","year":"1976"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.22151"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1128\/iai.29.1.252-260.1980","article-title":"Candida albicans: Ultrastructure: Colonization and invasion of oral epithelium","volume":"29","author":"Howlett JA","year":"1980","journal-title":"Infect Immun"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.2535\/ofaj1936.61.6_437"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1052150106"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(199605)245:1<9::AID-AR2>3.0.CO;2-V"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(199604)244:4<437::AID-AR2>3.0.CO;2-S"},{"key":"e_1_2_6_12_1","volume-title":"Microbiologia bucal","author":"Jorge AOC","year":"2007"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.12197"},{"key":"e_1_2_6_14_1","volume-title":"Oral microbiology","author":"Marsh PD","year":"2009"},{"key":"e_1_2_6_15_1","first-page":"11","article-title":"Scanning electron microscopy of the rat tongue mucosa with special attention to the bacteria on epithelial cell membranes","volume":"10","author":"Motoyama AA","year":"1999","journal-title":"Braz Dent J"},{"key":"e_1_2_6_16_1","first-page":"267","article-title":"Scanning electron microscopic study of the tongue mucosa in the Nine\u2010banded Armadillo. 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