{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:22:54Z","timestamp":1760660574445,"version":"build-2065373602"},"reference-count":39,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2013,10,9]],"date-time":"2013-10-09T00:00:00Z","timestamp":1381276800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Based on its histochemical properties, the secretory portion of the hamster submandibular gland has been classified as seromucous cells. The presence of endogenous peroxidase (PO) reaction was shown in the nuclear envelope, cisternae of endoplasmic reticulum and Golgi apparatus. The 3,3\u2032\u2010diaminobenzidene, tetrahydrochloride (DAB) method revealed bipartite secretory granules containing a PO\u2010positive dense core surrounded by a less dense halo in these cells. In the present investigation, serous and mucous\u2010like cells were found in resin\u2010embedded semi\u2010thin sections of the DAB\u2010reacted hamster submandibular gland. These sections were already on glass slides for routine light microscopic observations, therefore electron microscopic analysis could be unrealizable. We then used reflectance\u2010mode confocal laser scanning microscopy to visualize additional sites of PO activity as detected in these sections. Using this approach, we found mucous cells with PO activity\u2010negative secretory granules and seromucous cells with PO activity\u2010positive spot\u2010like secretory granules of the regular sublingual gland most frequently adjacent to the serous cells with typical electron\u2010dense secretory granules. These cells clearly differ from the seromucous cells with bipartite secretory granules and the granular duct cells with typical electron\u2010dense secretory granules of the hamster submandibular gland. Additionally, secretory endpieces of the ectopic sublingual gland\u2010like tissue empty into the duct of the hamster submandibular gland lobule. Thus, our findings suggest that a mass of sublingual gland tissue extends into the hamster submandibular gland during its development, and PO may be synthesized and secreted into the same duct. <jats:italic>Microsc. Res. Tech. 76:1284\u20131291, 2013<\/jats:italic>. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jemt.22298","type":"journal-article","created":{"date-parts":[[2013,10,11]],"date-time":"2013-10-11T22:43:19Z","timestamp":1381531399000},"page":"1284-1291","source":"Crossref","is-referenced-by-count":3,"title":["Confocal laser scanning microscopic analysis of ectopic sublingual gland\u2010like tissue inside the hamster submandibular gland"],"prefix":"10.1002","volume":"76","author":[{"given":"Keiichi","family":"Moriguchi","sequence":"first","affiliation":[{"name":"Department of Oral Anatomy School of Dentistry, Aichi\u2010Gakuin University Nagoya Aichi 464\u20108650 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michiya","family":"Utsumi","sequence":"additional","affiliation":[{"name":"Department of Oral Anatomy School of Dentistry, Aichi\u2010Gakuin University Nagoya Aichi 464\u20108650 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norikazu","family":"Ohno","sequence":"additional","affiliation":[{"name":"Department of Oral Anatomy School of Dentistry, Aichi\u2010Gakuin University Nagoya Aichi 464\u20108650 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,10,9]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00307102"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/10520299209110009"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.39.2.299"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(88)90210-2"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00277457"},{"key":"e_1_2_5_7_1","first-page":"317","article-title":"Oral salivary glands in golden hamster","volume":"102","author":"Boerner\u2010Patzelt D","year":"1956","journal-title":"Anat Anz"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00307787"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/14.4.291"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01462297"},{"key":"e_1_2_5_11_1","first-page":"807","article-title":"Laser scanning microscopy in enzyme histochemistry. 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