{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:28:34Z","timestamp":1760660914113,"version":"build-2065373602"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,11,6]],"date-time":"2013-11-06T00:00:00Z","timestamp":1383696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>We investigated the effect of leptin on the postnatal development of gap junctions between folliculo\u2010stellate cells by using Zucker fatty (fa\/fa) rats that have defects of the functional leptin receptor. Male Zucker fatty rats (fa\/fa) and male Zucker lean rats (+\/+) were used at each of the following postnatal ages: 20, 30, 40, 50, 60, 70, 80, 90 days, and 1 year. On one of the aforementioned dates, the anterior pituitary glands were prepared for observation by transmission electron microscopy. We quantified the number of follicles and gap junctions, and calculated the rate of occurrence as the ratio of the number of gap junctions existing between folliculo\u2010stellate cells per intersected follicular profile. In Zucker lean male rats, the number of gap junctions remained relatively constant from days 50 to 90 (0.44\u2009\u00b1\u20090.02 to 0.49\u2009\u00b1\u20090.03), and was similar in 1 year old rats (0.47\u2009\u00b1\u20090.03). These data were statistically higher compared to Zucker fatty male rats. In Zucker fatty male rats, very few gap junctions were observed in 30\u2010day\u2010old rats (0.04\u2009\u00b1\u20090.01: mean\u2009\u00b1\u2009SE). This disruption of gap junction formation persisted, and the number of gap junctions remained constant and showed a low level from days 40 to 90 (0.11\u2009\u00b1\u20090.02 to 0.17\u2009\u00b1\u20090.02); this finding was similar in 1\u2010year\u2010old rats (0.17\u2009\u00b1\u20090.02). These observations indicate that the effect of leptin over the gap junction formation within the anterior pituitary glands was directly mediated by interaction with the functional leptin receptor present on the folliculo\u2010stellate cells. <jats:italic>Microsc. Res. Tech. 77:31\u201336, 2014<\/jats:italic>. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jemt.22309","type":"journal-article","created":{"date-parts":[[2013,11,6]],"date-time":"2013-11-06T23:18:35Z","timestamp":1383779915000},"page":"31-36","source":"Crossref","is-referenced-by-count":2,"title":["The changes of gap junctions between pituitary folliculo\u2010stellate cells during the postnatal development of zucker fatty and lean rats"],"prefix":"10.1002","volume":"77","author":[{"given":"Eisuke","family":"Sakuma","sequence":"first","affiliation":[{"name":"Department of Functional Anatomy Nagoya City University Graduate School of Medical Sciences Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ikuo","family":"Wada","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Nagoya City University Graduate School of Medical Sciences Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsuyoshi","family":"Soji","sequence":"additional","affiliation":[{"name":"Department of Functional Anatomy Nagoya City University Graduate School of Medical Sciences Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenjiro","family":"Wakabayashi","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Nagoya City University Graduate School of Medical Sciences Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takanobu","family":"Otsuka","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Nagoya City University Graduate School of Medical Sciences Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Damon C.","family":"Herbert","sequence":"additional","affiliation":[{"name":"Department of Cellular and Structural Biology Dental School University of Texas Health Science Center at San Antonio  Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,11,6]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.48.2.272"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.74.2.605"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1385\/ENDO:22:3:203"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24096"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/27376"},{"key":"e_1_2_5_7_1","first-page":"225","article-title":"The extracellular matrix component laminin promotes gap junction formation in the rat anterior pituitary gland","volume":"208","author":"Horiguchi K","year":"2011","journal-title":"J Endocrinol"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc.62.205"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.142.5.1703"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-77-6-1053"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81282-9"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1981.61.4.829"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2007.07.006"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2001.281.6.R2048"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(84)84149-1"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1999.tb02547.x"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.57.1.66"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0596-18"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1530\/eje.0.1400468"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/1.2.93"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0185(20010201)262:2<169::AID-AR1026>3.0.CO;2-Z"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc.65.269"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc.66.229"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.22108"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.20596"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092260311"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1012978525492"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)60940-7"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-2760(01)00476-3"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90151-5"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00307293"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-0115(01)00370-6"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(82)90323-7"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)62161-0"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.25.580"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-111-3-977"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-90-5-1320"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjemt.22309","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.22309","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T16:13:02Z","timestamp":1760631182000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.22309"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,6]]},"references-count":36,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["10.1002\/jemt.22309"],"URL":"https:\/\/doi.org\/10.1002\/jemt.22309","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"type":"print","value":"1059-910X"},{"type":"electronic","value":"1097-0029"}],"subject":[],"published":{"date-parts":[[2013,11,6]]}}}