{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T01:32:41Z","timestamp":1783042361394,"version":"3.54.6"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,12,19]],"date-time":"2013-12-19T00:00:00Z","timestamp":1387411200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>This study investigated and compared the ultrastructural and chemical properties of representative biomaterials for ocular surface regeneration: a human amniotic membrane (AM) in a basal plate, a human AM in reflected chorion, a preserved AM, and a human corneo\u2010scleral tissue. Assessments of the morphological differences in the extracellular matrices were evaluated by hematoxylin\u2013eosin, Masson's trichrome (for total collagen), and picrosirius\u2010red (for newly synthesized collagen) staining. Assessments of the changes in the molecular structures and chemical compositions of the biomaterials for ocular surface regeneration were evaluated by Raman spectroscopy. A placental AM (52 %) was a dense and thick collagenous structure compared to a reflected AM (23 %). The spectroscopy did not obtain any structural information for a preserved AM. The cornea group (100 %, control) and sclera group (104 %) showed the collagen lamellae and interfibrillar spacing, and a slight inflammatory reaction with more fibrous and granulomatous tissues. There was a formation of newly synthesized collagen in a placental AM, while there were few collagen components in a reflected AM. Human AM tissues showed consistent Raman spectra and the characteristic collagen bands, similar to the corneal and scleral tissues. Therefore, these findings suggest that human placental AM and reflected AM are structurally suitable for scleral and corneal surface regeneration, respectively, while human placental or preserved AM and reflected AM are molecularly and chemically suitable for corneal and scleral surface regeneration, respectively. <jats:italic>Microsc. Res. Tech.  77:183\u2013188, 2014<\/jats:italic>. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jemt.22326","type":"journal-article","created":{"date-parts":[[2013,12,19]],"date-time":"2013-12-19T23:48:46Z","timestamp":1387496926000},"page":"183-188","source":"Crossref","is-referenced-by-count":13,"title":["Molecular and chemical investigations and comparisons of biomaterials for ocular surface regeneration"],"prefix":"10.1002","volume":"77","author":[{"given":"Yeon\u2010Hee","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology The Catholic University of Korea Kyonggi\u2010do 480\u2010717 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung\u2010Hyun","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Kyung Hee University Seoul 130\u2010701 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae 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