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Fasting increased the number of GnRHR\u2010immunoreactive (\u2010IR) cells in intact animals, whereas reduced the density of ESR1\u2010IR cells in OVX rabbits. Estrogen priming decreased the number of ESR1\u2010IR cells in fasted and OVX animals. Ovariectomy increased the number of ESR1\u2010IR cells in fed rabbits, but caused an opposite effect in both fed and fasted animals treated with estrogen. Fasting down regulated the mRNA levels for <jats:italic>ESR1<\/jats:italic> and <jats:italic>GnRHR<\/jats:italic>. Estrogen\u2010priming reduced the abundance for <jats:italic>ESR1<\/jats:italic> mRNA in both fed and fasted rabbits, and that for <jats:italic>GnRHR<\/jats:italic> in fasted rabbits. Ovariectomy halved <jats:italic>ESR1<\/jats:italic> mRNA levels independently of treatment and feeding condition, whereas increased (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009001) that for <jats:italic>GnRHR<\/jats:italic> in estrogen\u2010primed rabbits. In all rabbits, an LH surge occurred 30 min after GnRH injection but the lowest levels were found in intact fasted rabbits and the highest in fasted, estrogen\u2010primed animals. The LH profile was similar in intact and OVX rabbits and neither fasting nor estrogen priming modified it. In conclusion, fasting differentially modifies the ESR1 and GnRHR expression in the pituitary, depending on the presence of gonadal hormones, indicating complex interactions between metabolic signals and ovarian steroids. <jats:italic>Microsc. Res. Tech. 77:201\u2013210, 2014<\/jats:italic>. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jemt.22328","type":"journal-article","created":{"date-parts":[[2013,12,26]],"date-time":"2013-12-26T11:34:39Z","timestamp":1388057679000},"page":"201-210","source":"Crossref","is-referenced-by-count":6,"title":["Ovarian hormones and fasting differentially regulate pituitary receptors for estrogen and gonadotropin\u2010releasing hormone in rabbit female"],"prefix":"10.1002","volume":"77","author":[{"given":"F.","family":"Parillo","sequence":"first","affiliation":[{"name":"Scuola di Bioscienze e Medicina veterinaria Universit\u00e0 di Camerino 62024 Matelica Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Zerani","sequence":"additional","affiliation":[{"name":"Scuola di Bioscienze e Medicina veterinaria Universit\u00e0 di Camerino 62024 Matelica Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Maranesi","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze biopatologiche ed Igiene delle produzioni animali e alimentari Laboratorio di Biotecnologie fisiologiche, Sezione di Fisiologia veterinaria, Universit\u00e0 di Perugia 06126 Perugia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Dall'Aglio","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze biopatologiche ed Igiene delle produzioni animali e alimentari Laboratorio di Biotecnologie fisiologiche, Sezione di Fisiologia veterinaria, Universit\u00e0 di Perugia 06126 Perugia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Galeati","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze mediche veterinarie Universit\u00e0 di Bologna 40064 Ozzano Emilia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Brecchia","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze biopatologiche ed Igiene delle produzioni animali e alimentari Laboratorio di Biotecnologie fisiologiche, Sezione di Fisiologia veterinaria, Universit\u00e0 di Perugia 06126 Perugia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Boiti","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze biopatologiche ed Igiene delle produzioni animali e alimentari Laboratorio di Biotecnologie fisiologiche, Sezione di Fisiologia veterinaria, Universit\u00e0 di Perugia 06126 Perugia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Gonz\u00e1lez\u2010Mariscal","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Reproducci\u00f3n Animal CINVESTAV Universidad Aut\u00f3noma de Tlaxcala\u2010CINVESTAV  Tlaxcala Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,12,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/frne.2000.0198"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod57.2.415"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.131.5.2222"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2004-1099"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00485-X"},{"key":"e_1_2_6_7_1","unstructured":"BrecchiaG BonannoA GaleatiG Dall'AglioC diGrigoliA ParilloF BoitiC.2004.Effects of short\u2010 and long\u2010term fasting on the ovarian axis and reproductive performance of rabbit does. 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