{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:26:22Z","timestamp":1760660782010,"version":"build-2065373602"},"reference-count":48,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,8,5]],"date-time":"2014-08-05T00:00:00Z","timestamp":1407196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Background<\/jats:italic>: Nothing is known about huge clusters (HC) of embryonic stem cells (ESC) in human fetal organs (HFO). <jats:italic>Aim<\/jats:italic>: To know the status of HC\u2010ESC in HFO. <jats:italic>Methods<\/jats:italic>: Morphology and immunohistochemistry (IHC) in 32 HFO of 7\u201340 gestational weeks (GW). <jats:italic>Results<\/jats:italic>: HC\u2010ESC were seen in many HFO including central nervous system, spinal cords, spine, soft tissue, bone, skin, thyroid, lung, liver, pancreas, gall bladder, extrahepatic bile duct, adrenal, kidney, bladder, foregut, midgut, hindgut, female and male genital organs, and neurons. HC\u2010ESC's were composed of two populations depending on constituting cells. One were large cells with ample acidophilic cytoplasms with vesicular nuclei and nucleoli. The other were small cells with scant cytoplasm with hyperchromatic nuclei without nucleoli, resembling lymphocytes. The HC\u2010ESC were frequently showed neuronal differentiation. HC\u2010ESC were positive for NCAM, synaptophysin, NSE, chromogranin, PDGFRA, AFP, ErbB2, bcl\u20102, KIT, MET. They were negative for CD45, CD3, CD20, EMA, CEA, CA19\u20109, cytokeratin (CK) 7, CK8, CK18, CK19, MUC1, MUC2, MUC5AC, and MUC6. The mean Ki\u201067 labeling index (LI) was 13%\u2009\u00b1\u20097%. HC\u2010ESC showed a little glycogen but lacked mucins. These HC\u2010ESC were seen in 7\u201325 GW, and they were rarely seen in 26\u201340 GW. <jats:italic>Conclusions<\/jats:italic>: The morphology, IHC, and ontogeny of HC\u2010ESC were described. <jats:italic>Microsc. Res. Tech. 77:825\u2013831, 2014<\/jats:italic>. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jemt.22405","type":"journal-article","created":{"date-parts":[[2014,8,5]],"date-time":"2014-08-05T02:18:58Z","timestamp":1407205138000},"page":"825-831","source":"Crossref","is-referenced-by-count":3,"title":["Huge clusters of embryonic stem cells in human embryos: A morphologic study"],"prefix":"10.1002","volume":"77","author":[{"given":"Tadashi","family":"Terada","sequence":"first","affiliation":[{"name":"Department of Pathology Shizuoka City Shimizu Hospital Shizuoka 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