{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T16:12:32Z","timestamp":1760631152212,"version":"build-2065373602"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,10,28]],"date-time":"2014-10-28T00:00:00Z","timestamp":1414454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Vascular remodeling in the brain occurs as a plastic change following neural over\u2010activity. The auditory midbrain (or inferior colliculus, IC) is an ideal place to study sound\u2010induced vascular changes because it is the brain's most vascularized structure and it is tonotopically organized. However, its micro\u2010vascular pattern remains poorly understood. Since the IC is a sphere\u2010like structure, the histological assessment of vasculature could depend on the angle of sectioning. Here, we studied the effects of cutting the IC at different angles on microvascular assessment, specifically: micro\u2010vascular density and the shape of microvascular lumen. Photomicrographs were taken from 5 \u00b5m toluidine blue\u2010stained histological sections obtained at two angles of sectioning: (a) the conventional coronal sectioning, and (b) a novel \u201ctangential\u201d sectioning (tangential to the dorso\u2010medial surface of the IC). Results showed that the tangential sections, in comparison with the coronal sections, yielded (a) a higher count of micro\u2010vascular density and (b) a higher proportion of round\u2010shaped micro\u2010vascular lumens. This discrepancy in results between two cut angles is likely related to the spatial pattern of blood vessels supplying the IC. We propose that the tangential sectioning should be adopted as standard for the accurate study of microvasculature in the IC. <jats:italic>Microsc. Res. Tech. 78:105\u2013110, 2015<\/jats:italic>. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jemt.22450","type":"journal-article","created":{"date-parts":[[2014,10,28]],"date-time":"2014-10-28T01:17:14Z","timestamp":1414459034000},"page":"105-110","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of microvasculature at the auditory midbrain\u2013the benefits of sectioning at a tangential angle"],"prefix":"10.1002","volume":"78","author":[{"given":"Edmund Cheung","family":"So","sequence":"first","affiliation":[{"name":"Department of Anesthesia China Medical University\u2010 An Nan Hospital Tainan Taiwan"},{"name":"Department of Anesthesia China Medical University Taichung Taiwan"},{"name":"Department of Anesthesia Taishan Medical University Taian Shangdong People's Republic of China"},{"name":"Department of Anesthesia, Nanshan branch of Qilu Hospital Shangdong University  People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui\u2010Pin","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Nursing Tzu Hui Institute of Technology, Nanzhou Township Pingtung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chung Hsi","family":"Hsing","sequence":"additional","affiliation":[{"name":"Department of Anesthesia Chi Mei Medical Center Tainan Taiwan"},{"name":"Department of Anesthesia Taipei Medical University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng Nan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Physiology National Cheng Kung University Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Kai","family":"Chang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Athletics and Coaching Science National Taiwan Sport University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul W.F.","family":"Poon","sequence":"additional","affiliation":[{"name":"Department of Physiology National Cheng Kung University Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,10,28]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001860408"},{"key":"e_1_2_5_3_1","first-page":"I","article-title":"The vascular system of the cerebral cortex","volume":"59","author":"Bar T","year":"1980","journal-title":"Adv Anat Embryol Cell Biol"},{"key":"e_1_2_5_4_1","first-page":"20","article-title":"Patterns of vascularization in the developing cerebral cortex","volume":"100","author":"Bar T","year":"1983","journal-title":"Ciba Found Symp"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125.9.1591"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(87)90113-3"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1999.86.4.1211"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2012.03.031"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1983.44"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2006.12.040"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00315454"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1987.38"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(00)00075-7"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.20263"},{"key":"e_1_2_5_15_1","first-page":"125","article-title":"The local circulation of the living brain; 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