{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:24:19Z","timestamp":1760660659294,"version":"build-2065373602"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,7,24]],"date-time":"2015-07-24T00:00:00Z","timestamp":1437696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100011019","name":"NKFIH","doi-asserted-by":"crossref","award":["TECH-09-A2-2009-0134"],"award-info":[{"award-number":["TECH-09-A2-2009-0134"]}],"id":[{"id":"10.13039\/501100011019","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Nonlinear microscopy techniques are being increasingly used to perform <jats:italic>in vivo<\/jats:italic> studies in dermatology. These methods enable us to investigate the morphology and monitor the physiological process in the skin by the use of femtosecond lasers operating in the red, near\u2010infrared spectral range (680\u20131,300 nm). In this work we used two different techniques that require no labeling: second harmonic generation (SHG) for collagen detection and coherent anti\u2010Stokes Raman scattering (CARS) to assess lipid distribution in genetically obese murine skin. Obesity is one of the most serious public health problems due to its high and increasing prevalence and the associated risk of type 2 diabetes and cardiovascular diseases. Other than these diseases, nearly half of patients with diabetes mellitus suffer from dermatological complications such as delayed wound healing, foot ulcers and several other skin changes. In our experiment we investigated and followed the effects of obesity on dermal collagen alterations and adipocyte enlargement using a technique not reported in the literature so far. Our results indicate that the <jats:italic>in vivo<\/jats:italic> SHG and <jats:italic>ex vivo<\/jats:italic> CARS imaging technique might be an important tool for diagnosis of diabetes\u2010related skin disorders in the near future. <jats:italic>Microsc. Res. Tech. 78:823\u2013830, 2015<\/jats:italic>. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jemt.22545","type":"journal-article","created":{"date-parts":[[2015,7,24]],"date-time":"2015-07-24T07:39:57Z","timestamp":1437723597000},"page":"823-830","source":"Crossref","is-referenced-by-count":11,"title":["<i>In vivo<\/i> second\u2010harmonic generation and <i>ex vivo<\/i> coherent anti\u2010stokes raman scattering microscopy to study the effect of obesity to fibroblast cell function using an Yb\u2010fiber laser\u2010based <scp>CARS<\/scp> extension unit"],"prefix":"10.1002","volume":"78","author":[{"given":"D\u00f3ra","family":"Haluszka","sequence":"first","affiliation":[{"name":"Department of Dermatology Venereology and Dermatooncology, Semmelweis University Hungary Budapest Hungary"},{"name":"Department of Applied and Nonlinear Optics Institute for Solid State Physics and Optics Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kende","family":"L\u0151rincz","sequence":"additional","affiliation":[{"name":"Department of Dermatology Venereology and Dermatooncology, Semmelweis University Hungary Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00e1bor","family":"Moln\u00e1r","sequence":"additional","affiliation":[{"name":"MTA\u2010SZTE Research Group for Cortical Microcircuits Department of Physiology Anatomy and Neuroscience University of Szeged  Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00e1bor","family":"Tam\u00e1s","sequence":"additional","affiliation":[{"name":"MTA\u2010SZTE Research Group for Cortical Microcircuits Department of Physiology Anatomy and Neuroscience University of Szeged  Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Attila","family":"Kolonics","sequence":"additional","affiliation":[{"name":"Department of Applied and Nonlinear Optics Institute for Solid State Physics and Optics Budapest Hungary"},{"name":"R&amp;D Ultrafast Lasers Ltd Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u00f3bert","family":"Szip\u0151cs","sequence":"additional","affiliation":[{"name":"Department of Applied and Nonlinear Optics Institute for Solid State Physics and Optics Budapest Hungary"},{"name":"R&amp;D Ultrafast Lasers Ltd Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarolta","family":"K\u00e1rp\u00e1ti","sequence":"additional","affiliation":[{"name":"Department of Dermatology Venereology and Dermatooncology, Semmelweis University Hungary Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norbert M.","family":"Wikonk\u00e1l","sequence":"additional","affiliation":[{"name":"Department of Dermatology Venereology and Dermatooncology, Semmelweis University Hungary Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,7,24]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00340-011-4830-7"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamadermatol.2013.459"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2013.307"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.21082"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1364\/OE.18.007857"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2321027"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.anchem.1.031207.112754"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2011.145"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0846.2009.00364.x"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/lapl.200810098"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1165758"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1364\/OPEX.13.005791"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.6.1835"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M042515"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2011.01279.x"},{"key":"e_1_2_5_17_1","doi-asserted-by":"crossref","unstructured":"KolonicsA Cs\u00e1tiD AntalPG Szip\u0151csR.2012. A simple cost efficient fiber amplifier wavelength extension unit for broadly tunable femtosecond pulse Ti\u2010sapphire lasers for CARS microscopy. Biomedical Optics and 3D Imaging OSA Technical Digest (Optical Society of America) paper BSu3A.28.","DOI":"10.1364\/BIOMED.2012.BSu3A.28"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12464"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/35007508"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.4.000077"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1364\/OL.22.000528"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1364\/OE.17.020700"},{"key":"e_1_2_5_23_1","first-page":"641","volume-title":"Epidemiology of diabetic foot ulcers and amputation: Evidence for prevention. 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