{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T12:13:28Z","timestamp":1784204008069,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,10,24]],"date-time":"2015-10-24T00:00:00Z","timestamp":1445644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The aim of this study was to evaluate radiographically and histologically the pulpal and periapical response to self\u2010adhesive (Rely X\u2122 Unicem) and self\u2010etching and self\u2010curing (Multilink<jats:sup>\u00ae<\/jats:sup>) resin\u2010based luting materials in deep cavities in dogs' teeth. Deep class V cavities (0.5\u2010mm\u2013thick dentin) were prepared in 60 canine premolars and the following materials were applied on cavity floor: Groups I\/V\u2014RelyX\u2122 Unicem; Groups II\/VI\u2014Multilink<jats:sup>\u00ae<\/jats:sup>; Groups III\/VII\u2014zinc phosphate cement (control) and; Groups IV\/VIII\u2014gutta\u2010percha (control). Cavities were restored with silver amalgam. Animals were euthanized after 10 days (groups I\u2013IV) and 90 days (groups V\u2013VIII). Tooth\/bone blocks were radiographed and processed for histopathological evaluation of pulp and periapical tissue response to the materials. All materials presented similar histopathological features and radiographic findings at both periods. The pulp tissue was intact. The apical and periapical regions and periodontal ligament thickness were normal. No inflammatory cells, resorption of mineralized tissue (dentin, cementum, and alveolar bone) or bacteria were observed. The lamina dura was intact and no areas of periapical bone rarefaction or internal\/external root resorption were observed radiographically. It can be concluded that Rely X\u2122 Unicem and Multilink<jats:sup>\u00ae<\/jats:sup> caused no adverse tissue reactions and may be indicated for cementation of indirect restorations in deep dentin cavities without pulp exposure. <jats:italic>Microsc. Res. Tech. 78:1098\u20131103, 2015<\/jats:italic>. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jemt.22590","type":"journal-article","created":{"date-parts":[[2015,10,24]],"date-time":"2015-10-24T05:57:14Z","timestamp":1445666234000},"page":"1098-1103","source":"Crossref","is-referenced-by-count":6,"title":["Resin luting materials: Tissue response in dog's teeth"],"prefix":"10.1002","volume":"78","author":[{"given":"Osvaldo L.","family":"Bezzon","sequence":"first","affiliation":[{"name":"Department of Dental Materials and Prosthodontics School of Dentistry of Ribeir\u00e3o Preto, University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniella S. H.","family":"Rivera","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics Dental School, San Luis Potosi University San Luis Potosi Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel A. B.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela S. B.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Silva\u2010herzog","sequence":"additional","affiliation":[{"name":"Department of Endodontics Dental School, San Luis Potosi University San Luis Potosi Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo","family":"Nelson\u2010filho","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00edlia P.","family":"lucisano","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e9a A. B.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,10,24]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-005-0308-5"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.4012\/dmj.2014-167"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1067\/mpr.2003.20"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2008.10.011"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.2341\/12-417-L"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.2009.00623.x"},{"key":"e_1_2_5_8_1","first-page":"616.","article-title":"Cementation of porcelain restorations","volume":"14","author":"Blatz MB.","year":"2002","journal-title":"Pract Proced Aesthet Dent"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2005.10.007"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2014.02.006"},{"key":"e_1_2_5_11_1","first-page":"122","article-title":"Influence of different luting concepts on long term retentive strength of zirconia crowns","volume":"22","author":"Ernst CP","year":"2009","journal-title":"Am J Dent"},{"key":"e_1_2_5_12_1","first-page":"337","article-title":"Microtensile bond strength of self\u2010adhesive luting cements to ceramic","volume":"8","author":"Escribano N","year":"2006","journal-title":"J Adhes Dent"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-007-0162-8"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2007.11.021"},{"key":"e_1_2_5_15_1","unstructured":"International standardISO 7405:2008(E).2008. Preclinical evaluation of biocompatibility of medical devices used in dentistry\u2014Tests methods for dental materials."},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2012.04.037"},{"key":"e_1_2_5_17_1","volume-title":"Dentin and dentinogenesis","author":"Mj\u00f6r IA.","year":"1984"},{"key":"e_1_2_5_18_1","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1002\/jbm.b.31517","article-title":"In vitro biological response to a self\u2010adhesive resin cement under different curing strategies","volume":"92","author":"Monteiro GQ","year":"2010","journal-title":"J Biomed Mater Res B Appl Biomater"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820160307"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1708-8240.1998.tb00339.x"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2014.08.377"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(87)80014-6"},{"key":"e_1_2_5_23_1","first-page":"251","article-title":"Self\u2010adhesive resin cements: A literature review","volume":"10","author":"Radovic I","year":"2008","journal-title":"J Adhes Dent"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2008.08.002"},{"key":"e_1_2_5_25_1","first-page":"297","article-title":"Pulpal response to a newly developed MMA based resin cement for bonding tooth\u2010colored indirect restorations","volume":"19","author":"Seki Y","year":"2006","journal-title":"Am J Dent"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.2341\/07-101"},{"key":"e_1_2_5_27_1","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1055\/s-0039-1697418","article-title":"Cytotoxicity evaluation of self adhesive composite resin cements by dentin barrier test on 3D pulp cells","volume":"3","author":"Ulker HE","year":"2009","journal-title":"Eur J Dent"},{"key":"e_1_2_5_28_1","first-page":"596","article-title":"Interactions between cavity preparation and restoration events and their effects on pulp vitality","volume":"26","author":"Wisithphrom K","year":"2006","journal-title":"Int J Periodontics Restorative Dent"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjemt.22590","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.22590","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T14:26:37Z","timestamp":1760624797000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.22590"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,10,24]]},"references-count":27,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["10.1002\/jemt.22590"],"URL":"https:\/\/doi.org\/10.1002\/jemt.22590","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"value":"1059-910X","type":"print"},{"value":"1097-0029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,10,24]]}}}