{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T20:18:09Z","timestamp":1778185089038,"version":"3.51.4"},"reference-count":11,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,10,30]],"date-time":"2015-10-30T00:00:00Z","timestamp":1446163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Science and Technology Commission of Hangzhou Research","award":["20110833B13"],"award-info":[{"award-number":["20110833B13"]}]},{"name":"Science and Technology Commission of Hangzhou Research","award":["20092133W07"],"award-info":[{"award-number":["20092133W07"]}]},{"name":"Science and Technology Commission of Hangzhou Research","award":["20130733Q24"],"award-info":[{"award-number":["20130733Q24"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The use of noninvasive imaging techniques to evaluate different types of skin lesions is increasing popular. In vivo confocal laser scanning microscopy (CLSM) is a new method for high resolution non\u2010invasive imaging of intact skin in situ and in vivo. Although many studies have investigated melanin\u2010containing cells in lesions by in vivo CLSM, few studies have systematically characterized melanin\u2010containing cells based on their morphology, size, arrangement, density, borders, and brightness. In this study, the characteristics of melanin\u2010containing cells were further investigated by in vivo CLSM. A total of 130 lesions, including common nevi, giant congenital pigmented nevi, vitiligo, melasma, melanoma, and chronic eczema, were imaged by in vivo CLSM. This research helps dermatologists understand the characteristics of melanin\u2010containing cells and facilitate the clinical application of melanin\u2010containing cells in the investigation of dermatological disease. In summary, melanin\u2010containing cells include keratinocytes, melanocytes, macrophages, and melanocytic skin tumor cells. Our study presents the CLSM characteristics of melanin\u2010containing cells to potentially facilitate in vivo diagnosis based on shape, size, arrangement, density, borders, and brightness. <jats:italic>Microsc. Res. Tech. 78:1121\u20131127, 2015<\/jats:italic>. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jemt.22594","type":"journal-article","created":{"date-parts":[[2015,10,30]],"date-time":"2015-10-30T05:42:42Z","timestamp":1446183762000},"page":"1121-1127","source":"Crossref","is-referenced-by-count":8,"title":["Real\u2010time in vivo confocal laser scanning microscopy of melanin\u2010containing cells: A promising diagnostic intervention"],"prefix":"10.1002","volume":"78","author":[{"given":"Wenzhong","family":"Xiang","sequence":"first","affiliation":[{"name":"Department of Dermatology Third Hospital of Hangzhou, Affiliated Hangzhou Clinical College, Anhui Medical University Hangzhou 310009 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuzu","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Dermatology Third Hospital of Hangzhou, Affiliated Hangzhou Clinical College, Anhui Medical University Hangzhou 310009 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianzhong","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Dermatology Third Hospital of Hangzhou, Affiliated Hangzhou Clinical College, Anhui Medical University Hangzhou 310009 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aie","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Third Hospital of Hangzhou, Affiliated Hangzhou Clinical College, Anhui Medical University Hangzhou 310009 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhigang","family":"Bi","sequence":"additional","affiliation":[{"name":"Department of Dermatology the Affiliated BenQ Hospital of Nanjing Medical University Nanjing 210029 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,10,30]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2007.02275.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3880402"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/lapl.200810055"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21910"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1998.00309.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1001\/archdermatol.2009.388"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2009.00983.x"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.2009.01373.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/lapl.200710122"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800330"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-010-0764-2"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjemt.22594","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.22594","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T14:26:40Z","timestamp":1760624800000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.22594"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,10,30]]},"references-count":11,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["10.1002\/jemt.22594"],"URL":"https:\/\/doi.org\/10.1002\/jemt.22594","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"value":"1059-910X","type":"print"},{"value":"1097-0029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,10,30]]}}}