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Isolation of <jats:italic>Chlamydia suis<\/jats:italic> in cell culture is crucial for the generation and characterization of new isolates. However, isolation of <jats:italic>Chlamydia suis<\/jats:italic> strains from field samples is fastidious. Therefore, we exploited high\u2010content microscopy to quantify the growth of <jats:italic>Chlamydia suis<\/jats:italic> strains in different cell lines. We found that the cell line yielding optimal propagation of Chlamydia suis differed among isolates, and we identified cell lines outperforming those routinely used for chlamydial isolation. We conclude that adaptation of the propagation procedure to the origin of the putative field isolate is highly recommended to improve the recovery rate.<\/jats:p>","DOI":"10.1002\/jemt.22799","type":"journal-article","created":{"date-parts":[[2016,11,15]],"date-time":"2016-11-15T00:20:12Z","timestamp":1479169212000},"page":"350-356","source":"Crossref","is-referenced-by-count":17,"title":["Quantifying the growth of <i>chlamydia suis<\/i> in cell culture using high\u2010content microscopy"],"prefix":"10.1002","volume":"80","author":[{"given":"Leentje","family":"De Puysseleyr","sequence":"first","affiliation":[{"name":"Lab of Immunology and Animal Biotechnology, Faculty of Bioscience Engineering University of Ghent Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristien","family":"De Puysseleyr","sequence":"additional","affiliation":[{"name":"Lab of Immunology and Animal Biotechnology, Faculty of Bioscience Engineering University of Ghent Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisy","family":"Vanrompay","sequence":"additional","affiliation":[{"name":"Lab of Immunology and Animal Biotechnology, Faculty of Bioscience Engineering University of Ghent Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Winnok H.","family":"De Vos","sequence":"additional","affiliation":[{"name":"Lab of Cell Biology and Histology, Faculty of Veterinary Sciences University of Antwerp Antwerp Belgium"},{"name":"Cell Systems and Imaging, Faculty of Bioscience Engineering University of Ghent Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,11,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-51-1-203"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-014-0095-6"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-014-0560-x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0096704"},{"key":"e_1_2_7_6_1","article-title":"Assessment of Chlamydia suis Infection in Pig Farmers","author":"Puysseleyr L.","year":"2015","journal-title":"Transboundary and Emerging Diseases."},{"key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1002\/cyto.a.20807","article-title":"High content image cytometry in the context of subnuclear organization","volume":"77","author":"De Vos W. 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