{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T17:09:09Z","timestamp":1790356149035,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,12,9]],"date-time":"2016-12-09T00:00:00Z","timestamp":1481241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study evaluated the antibacterial activity of zinc oxide nanoparticles incorporated into self\u2010cured glass ionomer cement (GIC) and light\u2010cured resin\u2010reinforced GIC on <jats:italic>Streptococcus mutans<\/jats:italic> biofilm. The GICs, Fuji II (GC America) and Fuji II LC (GC America), were incorporated with nanoparticles at concentrations of 0%, 1%, and 2% by weight, and the biofilm maturation time was one and seven days. Circular specimens of each GIC type were prepared. The antibacterial activity was evaluated by determining the number of colony forming units of <jats:italic>S. mutans<\/jats:italic> strain per milliliter. Morphology of the biofilm was analyzed by scanning electron microscopy (SEM). The data obtained for each GIC were analyzed by two\u2010way ANOVA (\u03b1\u2009=\u20095%). For chemically activated GIC, no significant difference was observed in relation to the time of biofilm maturation (<jats:italic>p<\/jats:italic>\u2009=\u20090.744), concentration of nanoparticles (<jats:italic>p<\/jats:italic>\u2009=\u20090.966), and their interaction (<jats:italic>p<\/jats:italic>\u2009=\u20090.800). The results from analysis of GIC modified by light\u2010polymerized resin showed that only of the maturing time significantly affected the number of adhered cells on the biofilm (<jats:italic>p<\/jats:italic>\u2009=\u20090.034, <jats:italic>F<\/jats:italic>\u2009=\u20094.778). The more mature the biofilm, higher the number of cells. SEM analysis showed no change in cell morphology in relation to the type of GIC, maturation time, and nanoparticles concentration. We conclude that the inclusion of zinc oxide nanoparticles at concentrations of 1% and 2% by weight into the GICs evaluated here, did not promote their antimicrobial activity against <jats:italic>S. mutans<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/jemt.22814","type":"journal-article","created":{"date-parts":[[2016,12,9]],"date-time":"2016-12-09T15:05:48Z","timestamp":1481295948000},"page":"456-461","source":"Crossref","is-referenced-by-count":44,"title":["Antibacterial activity of glass ionomer cement modified by zinc oxide nanoparticles"],"prefix":"10.1002","volume":"80","author":[{"given":"Patr\u00edcia Petromilli Nordi Sasso","family":"Garcia","sequence":"first","affiliation":[{"name":"Department of Social Dentistry Araraquara School of Dentistry, University of S\u00e3o Paulo State S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Florian Bell","family":"Cardia","sequence":"additional","affiliation":[{"name":"Department of Social Dentistry Araraquara School of Dentistry, University of S\u00e3o Paulo State S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata Serignoli","family":"Francisconi","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathology Araraquara School of Dentistry, University of S\u00e3o Paulo State S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00edvia Nordi","family":"Dovigo","sequence":"additional","affiliation":[{"name":"Department of Social Dentistry Araraquara School of Dentistry, University of S\u00e3o Paulo State S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise Madalena Palomari","family":"Spolid\u00f3rio","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathology Araraquara School of Dentistry, University of S\u00e3o Paulo State S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra Nara","family":"de Souza Rastelli","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry Araraquara School of Dentistry University of S\u00e3o Paulo State S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Carolina","family":"Botta","sequence":"additional","affiliation":[{"name":"Department of General Dentistry Stony Brook School of Dental Medicine Stony Brook New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,12,9]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2008.06.002"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.03.003"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.07.003"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1010341107"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2009.03.004"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1806-83242005000200011"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2014.12.016"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.2217\/nnm.14.191"},{"key":"e_1_2_5_10_1","first-page":"636","article-title":"Inhibitory activity of glass\u2010ionomer cements on cariogenic bacteria","volume":"30","author":"Duque C.","year":"2005","journal-title":"Operative Dentistry"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2010.11.008"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2014.12.005"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2012.06.004"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2013.03.011"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.4103\/0972-0707.114358"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/la7035949"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.01012.x"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.5395\/rde.2014.39.2.109"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2009.04303.x"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2013.05.010"},{"key":"e_1_2_5_21_1","first-page":"411","article-title":"Antibacterial activity, fluoride release, and physical properties of an antibiotic\u2010modified glass ionomer cement","volume":"35","author":"Prabhakar A. R.","year":"2013","journal-title":"Pediatric Dentistry"},{"key":"e_1_2_5_22_1","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1002\/jbm.b.31620","article-title":"Antibacterial activity of dental composites containing zinc oxide nanoparticles","volume":"94","author":"Sevin\u00e7 B. A.","year":"2010","journal-title":"Journal of Biomedical Materials Research B: Applied Biomaterials"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmats.2015.00019"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.2319\/011408-19.1"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2013.12.001"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.2010.00770.x"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2011.02.006"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2011.03.016"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm8004897"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjemt.22814","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjemt.22814","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.22814","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T17:45:27Z","timestamp":1760636727000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.22814"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12,9]]},"references-count":28,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,5]]}},"alternative-id":["10.1002\/jemt.22814"],"URL":"https:\/\/doi.org\/10.1002\/jemt.22814","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"value":"1059-910X","type":"print"},{"value":"1097-0029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12,9]]}}}